Overview

  • Product name

  • Description

    Rabbit polyclonal to POLR1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human POLR1A aa 1425-1475. The exact sequence is proprietary.
    Database link: O95602

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: POLR1A IP in HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
  • Sequence similarities

    Belongs to the RNA polymerase beta' chain family.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • A190 antibody
    • AFDCIN antibody
    • DNA-directed RNA polymerase I largest subunit antibody
    • DNA-directed RNA polymerase I subunit A antibody
    • DNA-directed RNA polymerase I subunit rpa1 antibody
    • Polr1a antibody
    • Polymerase (RNA) I polypeptide A 194kDa antibody
    • RNA polymerase I 194 kDa subunit antibody
    • RNA polymerase I subunit A antibody
    • RNA polymerase I subunit A1 antibody
    • RPA1 antibody
    • RPA1_HUMAN antibody
    • RPA194 antibody
    • RPO1 4 antibody
    • RPO14 antibody
    see all

Images

  • All lanes : Anti-POLR1A antibody (ab241950) at 0.1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Exposure time: 3 minutes


    Prepared using NETN lysis buffer.

  • POLR1A was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

    ab241950 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.

    Lane 1: ab241950 IP in HeLa whole cell lysate.
    Lane 2: Control IgG.

    Chemiluminescence detection: 3 seconds.

References

ab241950 has not yet been referenced specifically in any publications.

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