Overview

  • Product name

    Anti-POLR1C antibody [EPR16178]
    See all POLR1C primary antibodies
  • Description

    Rabbit monoclonal [EPR16178] to POLR1C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human POLR1C aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: O15160

  • Positive control

    • K562, HepG2, 293, HeLa, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft.
  • Involvement in disease

    Treacher Collins syndrome 3 (TCS3) [MIM:248390]: A form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities

    Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 40kDa antibody
    • AA409007 antibody
    • AA959927 antibody
    • Ac2-127 antibody
    • AC40 antibody
    • AL024089 antibody
    • DNA directed RNA polymerase I subunit C antibody
    • DNA directed RNA polymerases I and III 40 kDa polypeptide antibody
    • DNA directed RNA polymerases I and III subunit RPAC1 antibody
    • DNA-directed RNA polymerase I subunit C antibody
    • DNA-directed RNA polymerases I and III 40 kDa polypeptide antibody
    • DNA-directed RNA polymerases I and III subunit RPAC1 antibody
    • MGC105583 antibody
    • MGC161175 antibody
    • POLR1C antibody
    • POLR1E antibody
    • Polymerase (RNA) I polypeptide C, 30kDa antibody
    • RNA polymerase 1 1 antibody
    • RNA polymerase I subunit C antibody
    • RNA polymerases I and III subunit AC1 antibody
    • RP3-337H4.4 antibody
    • RPA39 antibody
    • RPA40 antibody
    • RPA5 antibody
    • RPAC1 antibody
    • RPAC1_HUMAN antibody
    • RPC40 antibody
    • Rpo1 1 antibody
    • TCS3 antibody
    see all

Images

  • All lanes : Anti-POLR1C antibody [EPR16178] (ab196657) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 39 kDa

  • All lanes : Anti-POLR1C antibody [EPR16178] (ab196657) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 39 kDa

References

ab196657 has not yet been referenced specifically in any publications.

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