Overview

  • Product name
    Anti-POLR1D antibody
    See all POLR1D primary antibodies
  • Description
    Rabbit polyclonal to POLR1D
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-133 of Human POLR1D (AAH00889).

  • Positive control
    • A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, HCT116 cell lysate, HBC435 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab104115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 15 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively.
  • Involvement in disease
    Treacher Collins syndrome 2
  • Sequence similarities
    Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • AC19 antibody
    • DNA directed RNA polymerase I 16 kDa polypeptide antibody
    • DNA directed RNA polymerase I subunit D antibody
    • DNA directed RNA polymerases I and III subunit RPAC2 antibody
    • DNA-directed RNA polymerase I subunit D antibody
    • DNA-directed RNA polymerases I and III subunit RPAC2 antibody
    • FLJ20616 antibody
    • hRPA19 antibody
    • MGC9850 antibody
    • POLR1C antibody
    • POLR1D antibody
    • Polymerase (RNA) I polypeptide D antibody
    • Polymerase (RNA) I polypeptide D, 16kDa antibody
    • RNA polymerase I 16 kDa subunit antibody
    • RNA polymerases I and III subunit AC2 antibody
    • RPA16 antibody
    • RPA9 antibody
    • RPAC2 antibody
    • RPAC2_HUMAN antibody
    • RPC16 antibody
    • RPO1 3 antibody
    see all

Images

  • Western blot - Anti-POLR1D antibody (ab104115)
    Western blot - Anti-POLR1D antibody (ab104115)
    Anti-POLR1D antibody (ab104115) at 1/1000 dilution + HepG2 cell lysate at 30 µg

    Predicted band size: 15 kDa



    15% SDS-PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR1D antibody (ab104115)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR1D antibody (ab104115)
    ab104115, at 1/500 dilution, staining POLR1D in HBL435 xenograft by immunohistochemistry.

References

ab104115 has not yet been referenced specifically in any publications.

Publishing research using ab104115? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

There are currently no Customer reviews or Questions for ab104115.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up