Anti-POLR1D antibody (ab104115)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-POLR1D antibody
See all POLR1D primary antibodies -
Description
Rabbit polyclonal to POLR1D -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1-133 of Human POLR1D (AAH00889).
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Positive control
- A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, HCT116 cell lysate, HBC435 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab104115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 15 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Disease: Treacher Collins syndrome 2 Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. Similarity: Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family. -
Database links
- Entrez Gene: 539061 Cow
- Entrez Gene: 51082 Human
- Entrez Gene: 20018 Mouse
- Omim: 613715 Human
- SwissProt: Q1RMG8 Cow
- SwissProt: P0DPB5 Human
- SwissProt: P0DPB6 Human
- SwissProt: Q9Y2S0 Human
see all -
Alternative names
- AC19 antibody
- DNA-directed RNA polymerase I subunit D antibody
- DNA-directed RNA polymerases I and III subunit RPAC2 antibody
see all
Images
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Western blot - Anti-POLR1D antibody (ab104115)Anti-POLR1D antibody (ab104115) at 1/1000 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 15 kDa
15% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR1D antibody (ab104115)ab104115, at 1/500 dilution, staining POLR1D in HBL435 xenograft by immunohistochemistry.
References
ab104115 has not yet been referenced specifically in any publications.
