Anti-POLR1D antibody (ab104115)

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Western blot - Anti-POLR1D antibody (ab104115)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR1D antibody (ab104115)

Key features and details

  • Rabbit polyclonal to POLR1D
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-POLR1D antibody
    See all POLR1D primary antibodies

  • Description

    Rabbit polyclonal to POLR1D

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow

  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-133 of Human POLR1D (AAH00889).

  • Positive control

    • A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, HCT116 cell lysate, HBC435 cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab104115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 15 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Disease: Treacher Collins syndrome 2 Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. Similarity: Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

  • Database links

    see all

  • Alternative names

    • AC19 antibody
    • DNA-directed RNA polymerase I subunit D antibody
    • DNA-directed RNA polymerases I and III subunit RPAC2 antibody
    • hRPA19 antibody
    • POLR1D antibody
    • RNA polymerase I 16 kDa subunit antibody
    • RNA polymerases I and III subunit AC2 antibody
    see all

Images

  • Western blot - Anti-POLR1D antibody (ab104115)
    Western blot - Anti-POLR1D antibody (ab104115)
    Anti-POLR1D antibody (ab104115) at 1/1000 dilution + HepG2 cell lysate at 30 µg

    Predicted band size: 15 kDa



    15% SDS-PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR1D antibody (ab104115)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR1D antibody (ab104115)
    ab104115, at 1/500 dilution, staining POLR1D in HBL435 xenograft by immunohistochemistry.

References (1)

Publishing research using ab104115? Please let us know so that we can cite the reference in this datasheet.

ab104115 has been referenced in 1 publication.

  • Salva JE  et al. Nuclear FGFR2 regulates musculoskeletal integration within the developing limb. Dev Dyn 248:233-246 (2019). PubMed: 30620790

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