Product nameAnti-POLR1D antibody
See all POLR1D primary antibodies
DescriptionRabbit polyclonal to POLR1D
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human POLR1D aa 1-50. The exact sequence is proprietary.
Database link: Q9Y2S0
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to POLR1D immobilized on solid support.
Our Abpromise guarantee covers the use of ab241421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500.|
RelevanceDisease: Treacher Collins syndrome 2 Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. Similarity: Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.
- AC19 antibody
- DNA-directed RNA polymerase I subunit D antibody
- DNA-directed RNA polymerases I and III subunit RPAC2 antibody
All lanes : Anti-POLR1D antibody (ab241421) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
ab241421 has not yet been referenced specifically in any publications.