Anti-RPB3 antibody (ab138436)
Key features and details
- Rabbit polyclonal to RPB3
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-RPB3 antibody
See all RPB3 primary antibodies -
Description
Rabbit polyclonal to RPB3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human RPB3 aa 225-275.
Sequence:KPERFY YNVESCGSLR PETIVLSALS GLKKKLSDLQ TQLSHEIQSD VLTIN
Database link: NP_116558.1 -
Positive control
- WB: HeLa, Jurkat and 293T cell lysates. IP: HeLa cell lysates. IHC-P: Human breast carcinoma and mouse plasmacytoma tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99.01% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 31 kDa.
|
|
IP |
Use at 2-10 µg/mg of lysate.
|
|
IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 31 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. -
Sequence similarities
Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 415635 Chicken
- Entrez Gene: 5432 Human
- Entrez Gene: 20021 Mouse
- Entrez Gene: 361365 Rat
- Entrez Gene: 334669 Zebrafish
- Omim: 180663 Human
- SwissProt: P19387 Human
- SwissProt: P97760 Mouse
see all -
Alternative names
- DNA directed RNA polymerase II 33 kDa polypeptide antibody
- DNA directed RNA polymerase II subunit C antibody
- DNA directed RNA polymerase II subunit RPB3 antibody
see all
Images
-
All lanes : Anti-RPB3 antibody (ab138436) at 1/2000 dilution
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : 293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 3 minutes -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labelling RPB3 with ab138436 at a 1/1000 dilution.
-
Immunoprecipitation detection of RPB3 in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138436 at 6µg/mg lysate. Subsequent Western blot detection step was performed using ab138436 at 1µg/ml. Chemiluminescence with an exposure time of 10 seconds.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse plasmacytoma tissue labelling RPB3 with ab138436 at a 1/1000 dilution.
Protocols
Datasheets and documents
-
Datasheet download
References (2)
ab138436 has been referenced in 2 publications.
- Zhang P et al. Loss of ASXL1 in the bone marrow niche dysregulates hematopoietic stem and progenitor cell fates. Cell Discov 4:4 (2018). PubMed: 29423272
- Winton CE et al. A microchip platform for structural oncology applications. NPJ Breast Cancer 2:N/A (0). IP . PubMed: 27583302