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RecombinantRabMAb

Recombinant Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)

Reviews (1) Submit a question References (1)
Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
  • Flow Cytometry (Intracellular) - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
  • Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
  • Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11750] to POLR2E/rpb5
  • Suitable for: Flow Cyt (Intra), IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-POLR2E/rpb5 antibody [EPR11750]
    See all POLR2E/rpb5 primary antibodies

  • Description

    Rabbit monoclonal [EPR11750] to POLR2E/rpb5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human POLR2E/rpb5 aa 1-100. The exact sequence is proprietary.
    Database link: P19388

  • Positive control

    • HepG2, A431 and HeLa whole cell lysate (ab150035); Human kidney tissue; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Notes
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process.

  • Sequence similarities

    Belongs to the archaeal rpoH/eukaryotic RPB5 RNA polymerase subunit family.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • and III subunit ABC1 antibody
    • and III subunit RPABC1 antibody
    • DNA directed RNA polymerase II 23 kda polypeptide antibody
    • DNA directed RNA polymerase II 23 kDa polypeptide antibody
    • DNA directed RNA polymerase II polypeptide E antibody
    • DNA directed RNA polymerase II subunit E antibody
    • DNA directed RNA polymerases I II and III subunit RPABC1 antibody
    • DNA-directed RNA polymerase II 23 kDa polypeptide antibody
    • DNA-directed RNA polymerase II subunit E antibody
    • DNA-directed RNA polymerases I antibody
    • hRPB25 antibody
    • hsRPB5 antibody
    • II antibody
    • Polr2e antibody
    • Polymerase (RNA) II (DNA directed) polypeptide E (25kD) antibody
    • Polymerase (RNA) II (DNA directed) polypeptide E, 25kDa antibody
    • RNA polymerases I antibody
    • RNA polymerases I, II, and III subunit ABC1 antibody
    • RPAB1_HUMAN antibody
    • RPABC1 antibody
    • RPB5 antibody
    • RPB5 homolog antibody
    • XAP4 antibody
    see all

Images

  • Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    All lanes : Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151) at 1/20000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling POLR2E/rpb5 with ab180151 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Flow Cytometry (Intracellular) - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLR2E/rpb5 with ab180151 at 1/10 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green). 

     

     

  • Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151) at 1/5000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 25 kDa

  • Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)
    Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151)

References (1)

Publishing research using ab180151? Please let us know so that we can cite the reference in this datasheet.

ab180151 has been referenced in 1 publication.

  • Lee YL  et al. MAF1 represses CDKN1A through a Pol III-dependent mechanism. Elife 4:e06283 (2015). ChIP ; Human . PubMed: 26067234

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-POLR2E [EPR11750] antibody

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
African Green Monkey Cell (COS7)
Specification
COS7
Permeabilization
Yes - Triton X100 0.3%
Fixative
Formaldehyde
Read More

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Verified customer

Submitted May 02 2014

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