Anti-POLR2F antibody [2780C3a] (ab51362)
Key features and details
- Mouse monoclonal [2780C3a] to POLR2F
- Suitable for: WB
- Isotype: IgG1
Overview
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Product name
Anti-POLR2F antibody [2780C3a] -
Description
Mouse monoclonal [2780C3a] to POLR2F -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Immunogen
Recombinant fragment (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
2780C3a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. |
Target
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Relevance
POLR2F belongs to the archaeal rpoK/eukaryotic RPB6 RNA polymerase subunit family and is the sixth largest subunit of RNA polymerase II. This polymerase, along with two other DNA-directed RNA polymerases, is responsible for synthesizing messenger RNA in eukaryotes. Three distinct zinc-containing RNA polymerases are found in eukaryotic nuclei: polymerase I for the ribosomal RNA precursor, polymerase II for the mRNA precursor, and polymerase III for 5S and tRNA genes. Each class of RNA polymerase is assembled from 9 to 15 different polypeptides. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. -
Cellular localization
Nuclear -
Alternative names
- DNA directed RNA polymerase II 14.4 kda polypeptide antibody
- DNA directed RNA polymerase II polypeptide F antibody
- DNA directed RNA polymerases I, II, and III 14.4 kDa polypeptide antibody
see all
Images
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Anti-POLR2F antibody [2780C3a] (ab51362) + immunizing peptide
Predicted band size: 14 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab51362 has not yet been referenced specifically in any publications.