Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityProtein G purified
Purification notesFiltered through a 0.22 µm membrane.
Our Abpromise guarantee covers the use of ab51362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.|
RelevancePOLR2F belongs to the archaeal rpoK/eukaryotic RPB6 RNA polymerase subunit family and is the sixth largest subunit of RNA polymerase II. This polymerase, along with two other DNA-directed RNA polymerases, is responsible for synthesizing messenger RNA in eukaryotes. Three distinct zinc-containing RNA polymerases are found in eukaryotic nuclei: polymerase I for the ribosomal RNA precursor, polymerase II for the mRNA precursor, and polymerase III for 5S and tRNA genes. Each class of RNA polymerase is assembled from 9 to 15 different polypeptides. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template.
- DNA directed RNA polymerase II 14.4 kda polypeptide antibody
- DNA directed RNA polymerase II polypeptide F antibody
- DNA directed RNA polymerases I, II, and III 14.4 kDa polypeptide antibody
Anti-POLR2F antibody [2780C3a] (ab51362) + immunizing peptide
Predicted band size: 14 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab51362 has not yet been referenced specifically in any publications.