Anti-POLR2G antibody - C-terminal (ab170847)

Overview

  • Product name
    Anti-POLR2G antibody - C-terminal
    See all POLR2G primary antibodies
  • Description
    Rabbit polyclonal to POLR2G - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human POLR2G aa 145-153 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P62487

  • Positive control
    • Jurkat cells and cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab170847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA.
  • Sequence similarities
    Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA directed RNA polymerase II 19 kDa polypeptide antibody
    • DNA directed RNA polymerase II subunit G antibody
    • DNA directed RNA polymerase II subunit RPB7 antibody
    • DNA-directed RNA polymerase II subunit G antibody
    • DNA-directed RNA polymerase II subunit RPB7 antibody
    • hRPB19 antibody
    • hsRPB7 antibody
    • MGC138367 antibody
    • MGC138369 antibody
    • POLR2G antibody
    • polymerase (RNA) II (DNA directed) polypeptide G antibody
    • RNA polymerase II 19 kDa subunit antibody
    • RNA polymerase II subunit B7 antibody
    • RPB19 antibody
    • RPB7 antibody
    • RPB7_HUMAN antibody
    see all

Images

  • Flow cytometric analysis of Jurkat cells labeling POLR2G using ab170847 at a 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-POLR2G antibody - C-terminal (ab170847) at 1/100 dilution + Jurkat cell line lysate at 35 µg

    Predicted band size: 19 kDa

References

ab170847 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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