Anti-POLR2I antibody (ab192407)
Key features and details
- Rabbit polyclonal to POLR2I
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-POLR2I antibody
See all POLR2I primary antibodies -
Description
Rabbit polyclonal to POLR2I -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human POLR2I. The exact sequence is proprietary.
Database link: P36954 -
Positive control
- A549, NIH 3T3 and PC12 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab192407 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa. |
Target
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Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template. -
Sequence similarities
Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
Contains 1 TFIIS-type zinc finger. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5438 Human
- Entrez Gene: 69920 Mouse
- Entrez Gene: 292778 Rat
- Omim: 180662 Human
- SwissProt: P36954 Human
- SwissProt: P60898 Mouse
- Unigene: 47062 Human
- Unigene: 440028 Mouse
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Alternative names
- DNA directed RNA polymerase II 14.5 kDa polypeptide antibody
- DNA directed RNA polymerase II polypeptide I antibody
- DNA directed RNA polymerase II subunit I antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab192407 has not yet been referenced specifically in any publications.