Key features and details
- Rabbit polyclonal to POLR3A
- Suitable for: ChIP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-POLR3A antibody
See all POLR3A primary antibodies
DescriptionRabbit polyclonal to POLR3A
Tested applicationsSuitable for: ChIP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chicken, Cow
Recombinant fragment corresponding to Human POLR3A aa 392-632.
FPEKVNKANINFLRKLVQNGPEVHPGANFIQQRHTQMKRFLKYGNREKMA QELKYGDIVERHLIDGDVVLFNRQPSLHKLSIMAHLARVKPHRTFRFNEC VCTPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAA IQDFLTGAYLLTLKDTFFDRAKACQIIASILVGKDEKIKVRLPPPTILKP VTLWTGKQIFSVILRPSDDNPVRANLRTKGKQYCGKGEDLC
Database link: O14802
- IHC-P: Human testis and small intestine tissue. ChIP: HEK-293T cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab238596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|IHC-P||1/20 - 1/200.|
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Tissue specificityExpressed in the brain, in the cortex and the white matter (at protein level).
Involvement in diseaseLeukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism
Sequence similaritiesBelongs to the RNA polymerase beta' chain family.
- Information by UniProt
- BC053071 antibody
- DNA directed RNA polymerase III largest subunit antibody
- DNA directed RNA polymerase III subunit A antibody
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells (1.6 x 106) were cross-linked with formaldehyde, sonicated and immunoprecipitated with 4 µg of ab238596 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the tRNA-Leu Anti-Codon (TAG) promoter.
Paraffin-embedded human testis tissue stained for POLR3A with ab238596 at a 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for POLR3A with ab238596 at a 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238596 has not yet been referenced specifically in any publications.