Anti-POLR3A antibody (ab96328)
Key features and details
- Rabbit polyclonal to POLR3A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-POLR3A antibody
See all POLR3A primary antibodies -
Description
Rabbit polyclonal to POLR3A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 182 and 452 of POLR3A
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 156 kDa.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 156 kDa. |
Target
-
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. -
Tissue specificity
Expressed in the brain, in the cortex and the white matter (at protein level). -
Involvement in disease
Leukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism -
Sequence similarities
Belongs to the RNA polymerase beta' chain family. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 11128 Human
- Omim: 614258 Human
- SwissProt: O14802 Human
- Unigene: 436896 Human
-
Alternative names
- BC053071 antibody
- DNA directed RNA polymerase III largest subunit antibody
- DNA directed RNA polymerase III subunit A antibody
see all
Images
-
Western blot - Anti-POLR3A antibody (ab96328)Anti-POLR3A antibody (ab96328) at 1/500 dilution + HCT116 cell lysate at 30 µg
Predicted band size: 156 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab96328 has been referenced in 8 publications.
- Choquet K et al. Leukodystrophy-associated POLR3A mutations down-regulate the RNA polymerase III transcript and important regulatory RNA BC200. J Biol Chem 294:7445-7459 (2019). PubMed: 30898877
- Frischknecht L et al. BRAF inhibition sensitizes melanoma cells to a-amanitin via decreased RNA polymerase II assembly. Sci Rep 9:7779 (2019). PubMed: 31123282
- Gilbertson S et al. Changes in mRNA abundance drive shuttling of RNA binding proteins, linking cytoplasmic RNA degradation to transcription. Elife 7:N/A (2018). PubMed: 30281021
- Karijolich J et al. Genome-wide mapping of infection-induced SINE RNAs reveals a role in selective mRNA export. Nucleic Acids Res 45:6194-6208 (2017). ChIP . PubMed: 28334904
- Choquet K et al. Absence of neurological abnormalities in mice homozygous for the Polr3a G672E hypomyelinating leukodystrophy mutation. Mol Brain 10:13 (2017). PubMed: 28407788
- Ogunjimi B et al. Inborn errors in RNA polymerase III underlie severe varicella zoster virus infections. J Clin Invest 127:3543-3556 (2017). PubMed: 28783042
- Lee YL et al. MAF1 represses CDKN1A through a Pol III-dependent mechanism. Elife 4:e06283 (2015). ChIP, WB ; Human . PubMed: 26067234
- Fontenot E et al. A novel monoclonal antibody to secreted frizzled-related protein 2 inhibits tumor growth. Mol Cancer Ther 12:685-95 (2013). PubMed: 23604067