Key features and details
- Rabbit polyclonal to POLR3A - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-POLR3A antibody - N-terminal
See all POLR3A primary antibodies
DescriptionRabbit polyclonal to POLR3A - N-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from the N terminal of Human POLR3A
- 293 cell lysate
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab138023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 156 kDa.|
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Tissue specificityExpressed in the brain, in the cortex and the white matter (at protein level).
Involvement in diseaseLeukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism
Sequence similaritiesBelongs to the RNA polymerase beta' chain family.
- Information by UniProt
- BC053071 antibody
- DNA directed RNA polymerase III largest subunit antibody
- DNA directed RNA polymerase III subunit A antibody
ab138023 has not yet been referenced specifically in any publications.