Overview

  • Product name

    Anti-POLR3A antibody - N-terminal
    See all POLR3A primary antibodies
  • Description

    Rabbit polyclonal to POLR3A - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the N terminal of Human POLR3A

  • Positive control

    • 293 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab138023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 156 kDa.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • Tissue specificity

    Expressed in the brain, in the cortex and the white matter (at protein level).
  • Involvement in disease

    Leukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism
  • Sequence similarities

    Belongs to the RNA polymerase beta' chain family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BC053071 antibody
    • DNA directed RNA polymerase III largest subunit antibody
    • DNA directed RNA polymerase III subunit A antibody
    • DNA-directed RNA polymerase III largest subunit antibody
    • DNA-directed RNA polymerase III subunit A antibody
    • DNA-directed RNA polymerase III subunit RPC1 antibody
    • hRPC155 antibody
    • MGC62420 antibody
    • POLR 3A antibody
    • POLR3A antibody
    • Polymerase (RNA) III (DNA directed) polypeptide A 155kDa antibody
    • Polymerase (RNA) III (DNA directed) polypeptide A antibody
    • RGD1305574 antibody
    • RNA polymerase III 155 kDa subunit antibody
    • RNA polymerase III subunit C1 antibody
    • RNA polymerase III subunit C160 antibody
    • RNA polymerase III subunit RPC155 D antibody
    • RPC1 antibody
    • RPC1_HUMAN antibody
    • RPC155 antibody
    see all

Images

  • Anti-POLR3A antibody - N-terminal (ab138023) at 1/500 dilution + 293 cell lysate at 30 µg

    Predicted band size: 156 kDa

References

ab138023 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa S3)
Specification
HeLa S3
Fixative
Formaldehyde
Permeabilization
Yes - Triton 0.3%
Blocking step
Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 29 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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