Overview

  • Product name
    Anti-POLR3B antibody [EPR8719]
    See all POLR3B primary antibodies
  • Description
    Rabbit monoclonal [EPR8719] to POLR3B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human POLR3B (UniProt ID: Q9NW08)

  • Positive control
    • Hela or HepG2 cell lysates; 293T cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 128 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
    • Sequence similarities
      Belongs to the RNA polymerase beta chain family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C128 antibody
      • DNA directed RNA polymerase III 127.6 kDa polypeptide antibody
      • DNA directed RNA polymerase III subunit B antibody
      • DNA-directed RNA polymerase III 127.6 kDa polypeptide antibody
      • DNA-directed RNA polymerase III subunit B antibody
      • DNA-directed RNA polymerase III subunit RPC2 antibody
      • FLJ10388 antibody
      • polr3b antibody
      • Polymerase (RNA) III (DNA directed) polypeptide B antibody
      • RNA polymerase III subunit C2 antibody
      • RNA polymerase III subunit RPC2 antibody
      • RPC2 antibody
      • RPC2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-POLR3B antibody [EPR8719] (ab137030) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Hela cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 128 kDa

    • Flow cytometry analysis of permeabilized 293T cells labelling POLR3B with ab137030 at 1/10 dilution (red) or a negative rabbit IgG (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab137030 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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