Product nameAnti-POLR3D antibody
DescriptionRabbit polyclonal to POLR3D
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide corresponding to a region between residues 175 and 225 of human POLR3D (NP_001713.2).
- HeLa and 293T whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab86786 was affinity purified using an epitope specific to POLR3D immobilized on solid support.
Our Abpromise guarantee covers the use of ab86786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 44 kDa.|
|IP||Use at 10 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevancePOLR3D is a DNA dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling POLR3D with ab86786 at 1/1000 (0.2µg/ml). Detection: DAB.
All lanes : Anti-POLR3D antibody (ab86786) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 3 minutes
Detection of POLR3D by Western Blot of Immunprecipitate.
ab86786, at 1 µg/ml, staining POLR3D in HeLa whole cell lysate immunoprecipitated using ab86786 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 3 seconds.
This product has been referenced in:
- Van Bortle K et al. Topological organization and dynamic regulation of human tRNA genes during macrophage differentiation. Genome Biol 18:180 (2017). Read more (PubMed: 28931413) »
- Wang J et al. Cytoskeletal Filamin A Differentially Modulates RNA Polymerase III Gene Transcription in Transformed Cell Lines. J Biol Chem 291:25239-25246 (2016). Read more (PubMed: 27738102) »