Overview

  • Product name

  • Description

    Rabbit polyclonal to POLR3E
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 475-525 of Human POLR3E (NP_060589.1).

  • Positive control

    • Whole cell lysate from HeLa, 293T and Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab134560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    DNA dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. There are three isoforms.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • DNA directed RNA polymerase III 80 kDa polypeptide antibody
    • DNA-directed RNA polymerase III subunit RPC5 antibody
    • FLJ10509 antibody
    • KIAA1452 antibody
    • polymerase (RNA) III (DNA directed) polypeptide E (80kD) antibody
    • Polymerase (RNA) III (DNA directed) polypeptide E antibody
    • RNA polymerase III 80 kDa subunit RPC5 antibody
    • RNA polymerase III subunit C5 antibody
    • RPC5 antibody
    • SIN antibody
    see all

Images

  • All lanes : Anti-POLR3E antibody (ab134560) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 80 kDa


    Exposure time: 30 seconds
  • Detection of POLR3E by Western Blot of Immunprecipitate.
    Immunoprecipitation of POLR3E in HeLa whole cell lysate, using ab134560 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    For the subsequent WB, ab134560 was used at 1 µg/ml.
    The lysate in lane 2 is precipitated using an IgG control antibody.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab134560 has not yet been referenced specifically in any publications.

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