Anti-RPC39 antibody (ab76951)
Key features and details
- Rabbit polyclonal to RPC39
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPC39 antibody
See all RPC39 primary antibodies -
Description
Rabbit polyclonal to RPC39 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human RPC39 (N terminal).
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Positive control
- Human brain tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa.
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 2.5 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa. |
ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. -
Sequence similarities
Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10621 Human
- SwissProt: Q9H1D9 Human
- Unigene: 472227 Human
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Alternative names
- DNA directed RNA polymerase III 39 kDa polypeptide antibody
- DNA directed RNA polymerase III 39 kDa polypeptide F antibody
- DNA directed RNA polymerase III subunit F antibody
see all
Images
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Immunofluorescence of RPC39 in Human brain tissue with ab76951 at 20 ug/mL.
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Lane 1 : Anti-RPC39 antibody (ab76951) at 0.5 µg/ml
Lane 2 : Anti-RPC39 antibody (ab76951) at 1 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 36 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
ab76951 at 2.5µg/ml staining RPC39 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab76951 has not yet been referenced specifically in any publications.