Overview

  • Product name
  • Description
    Rabbit polyclonal to POLR3G
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Monkey
  • Immunogen

    Synthetic peptide within Human POLR3G (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: O15318

  • Positive control
    • WB: MCF7, RAW 264.7 and H9c2 whole cell lysates. IHC-P: Human tonsil and brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab230854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 26 kDa.

Target

  • Function
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • Sequence similarities
    Belongs to the eukaryotic RPC7 RNA polymerase subunit family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA directed RNA polymerase III 32 kDa polypeptide antibody
    • DNA-directed RNA polymerase III subunit G antibody
    • DNA-directed RNA polymerase III subunit RPC7 antibody
    • Polr3g antibody
    • Polymerase (RNA) III (DNA directed) polypeptide G (32kD) antibody
    • RNA polymerase III 32 kDa subunit antibody
    • RNA polymerase III subunit C7 antibody
    • RPC32 antibody
    • RPC7 antibody
    • RPC7_HUMAN antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for POLR3G using ab230854 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human brain tissue stained for POLR3G using ab230854 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-POLR3G antibody (ab230854) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : H9c2 (rat heart myoblast cell line) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 26 kDa

References

ab230854 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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