Key features and details
- Rabbit polyclonal to POLR3K
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-POLR3K antibody
DescriptionRabbit polyclonal to POLR3K
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to amino acids 6-108 of Human POLR3K
- Human testis tissue; Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6.0|
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Sequence similaritiesBelongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
Contains 1 TFIIS-type zinc finger.
- Information by UniProt
- C11 antibody
- C11 RNP3 antibody
- DNA directed RNA polymerase III subunit K antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab121238 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-POLR3K antibody (ab121238) at 1/250 dilution
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemical staining of paraffin embedded Human testis tissue, using 1/50 ab121238, shows strong nuclear positivity in cells of seminiferus ducts.
ab121238 has not yet been referenced specifically in any publications.