Key features and details
- Rabbit polyclonal to POLRMT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-POLRMT antibody
See all POLRMT primary antibodies
DescriptionRabbit polyclonal to POLRMT
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human POLRMT (internal sequence). The exact sequence is proprietary.
Database link: O00411
- ICC/IF: HepG2 cells. IHC-P: Human hepatoma tissue. WB: HepG2 whole cell lysate.
This product was previously labelled as mtRNA polymerase
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 139 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionDNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.
Sequence similaritiesBelongs to the phage and mitochondrial RNA polymerase family.
- Information by UniProt
- APOLMT antibody
- DNA-directed RNA polymerase antibody
- DNA-directed RNA polymerase mitochondrial antibody
Anti-POLRMT antibody (ab228576) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 139 kDa
Methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for POLRMT (green) using ab228576 (1/500 dilution) in ICC/IF.
Counterstain: Hoechst 33342 (blue).
Paraffin-embedded human hepatoma tissue stained for POLRMT with ab228576 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228576 has not yet been referenced specifically in any publications.