Overview

  • Product name

  • Description

    Rabbit polyclonal to POLRMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human POLRMT (internal sequence). The exact sequence is proprietary.
    Database link: O00411

  • Positive control

    • ICC/IF: HepG2 cells. IHC-P: Human hepatoma tissue. WB: HepG2 whole cell lysate.
  • General notes

     This product was previously labelled as mtRNA polymerase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab228576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 139 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.
  • Sequence similarities

    Belongs to the phage and mitochondrial RNA polymerase family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • APOLMT antibody
    • DNA-directed RNA polymerase antibody
    • DNA-directed RNA polymerase mitochondrial antibody
    • h-mtRPOL antibody
    • mitochondrial antibody
    • MTRNAP antibody
    • MtRPOL antibody
    • POLRMT antibody
    • polymerase (RNA) mitochondrial (DNA directed) antibody
    • polymerase, RNA, mitochondrial antibody
    • RPOM antibody
    • RPOM_HUMAN antibody
    see all

Images

  • Anti-POLRMT antibody (ab228576) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 139 kDa



    5% SDS-PAGE

  • Methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for POLRMT (green) using ab228576 (1/500 dilution) in ICC/IF.

    Counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded human hepatoma tissue stained for POLRMT with ab228576 at 1/500 dilution in immunohistochemical analysis.

References

ab228576 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228576.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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