Overview

  • Product name

  • Description

    Rabbit polyclonal to POLRMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human POLRMT aa 1200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab32987)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates; HeLa, HEK293 and Caco2.
  • General notes

     This product was previously labelled as mtRNA polymerase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab32988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 139 kDa).

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.
  • Sequence similarities

    Belongs to the phage and mitochondrial RNA polymerase family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • APOLMT antibody
    • DNA-directed RNA polymerase antibody
    • DNA-directed RNA polymerase mitochondrial antibody
    • h-mtRPOL antibody
    • mitochondrial antibody
    • MTRNAP antibody
    • MtRPOL antibody
    • POLRMT antibody
    • polymerase (RNA) mitochondrial (DNA directed) antibody
    • polymerase, RNA, mitochondrial antibody
    • RPOM antibody
    • RPOM_HUMAN antibody
    see all

Images

  • All lanes : Anti-POLRMT antibody (ab32988) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 139 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 160 kDa, 45 kDa, 65 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Herbers E  et al. Tissue specific differences in mitochondrial DNA maintenance and expression. Mitochondrion N/A:N/A (2018). WB . Read more (PubMed: 29339192) »
  • Nouws J  et al. Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import. J Biol Chem 291:989-97 (2016). Read more (PubMed: 26586915) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Vielen Dank für Ihren Anruf vom letzten Freitag.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten.

Wir haben nunalle Röhrchen die wir an Lager haben überprüft. In keinem unserer Röhrchen hierhatte es ein Präzipitat. Das Problem scheintnur das eine Röhrchen betroffen zu haben, und wir können Ihnen daher ein Ersatzröhrchen senden. Ich werde Ihnen deshalb wie am Telefon besprochen,ein kostenloses Ersatzröhrchen senden.Diese Sendung hat die Referenznummer x und sollteMittwoch bei Ihnen ankommen. Das neue Röhrchen ist natürlich auch von unserer Garantie abgedeckt.

Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Question
Answer

Thank you for contacting us.

As discussed please remove the precipitates by centrifuging the vial and use it in western blot. If the antibody fails please do not hesitate to contact me for replacement.

Have a nice day!

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