Overview

  • Product name
    Anti-Poly (ADP-Ribose) Polymer antibody
    See all Poly (ADP-Ribose) Polymer primary antibodies
  • Description
    Chicken polyclonal to Poly (ADP-Ribose) Polymer
  • Host species
    Chicken
  • Specificity
    This antibody recognizes Poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP-ribose) polymerases (PARP)-related enzymes, including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. It does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. It does cross-react to bovine serum albumin due to its use as a carrier for the immunogen.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Predicted to react with a wide range of species due to sequence homology.
  • Immunogen

    Other Immunogen Type corresponding to Poly (ADP-Ribose) Polymer. Purified Poly (ADP-Ribose) Polymer mixed with methylated bovine serum albumin.

Properties

Applications

Our Abpromise guarantee covers the use of ab14460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-Fr Use at an assay dependent dilution.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 1 kDa.
ELISA Use at an assay dependent dilution. It is recommended to start with a 1/100 dilution.
IHC-P Use at an assay dependent dilution.

Target

  • Relevance
    Poly (ADP-Ribose) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer poly (ADP-Ribose), with chain lengths ranging from 2 to 300 residues, containing approximately 2% branching in the chain. Poly (ADP-Ribose) polymer becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of poly (ADP-Ribose) polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).
  • Alternative names
    • pADPr antibody
    • Poly ADP ribose antibody

Images

  • Poly (ADP-Ribose) Polymer staining of untreated rat liver, with ab14460 at a concentration of 20µg/ml.

  • Western blot analysis of Poly (ADP-Ribose) Polymer. Lane 1: control HL60 cells (75,000 cells). Lane 2: cells automodified with PARP1. Note the apparent shift in molecular weight PARP1 from 116kDa to a broad range >250kDa due to various extents of Poly (ADP-Ribose) Polymer automodification. ab14460 was used at a concentration of 10 µg/ml.

  • Poly (ADP-Ribose) Polymer staining of livers from rats injected with diethylnitrosamine (200 mg/kg). The livers were removed and rapidly processed 10 hr later, at peak polymer induction. ab14460 was used at a concentration of 20µg/ml

  • ab14460 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C.  A Cy5® conjugated donkey anti-chicken antibody diluted 1/300 was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Horton JK  et al. Role of the oxidized form of XRCC1 in protection against extreme oxidative stress. Free Radic Biol Med 107:292-300 (2017). Read more (PubMed: 28179111) »
  • Horton JK  et al. DNA polymerase ß-dependent cell survival independent of XRCC1 expression. DNA Repair (Amst) 26:23-9 (2015). Read more (PubMed: 25541391) »
See all 7 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Ovarian cancer cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Treatment
PARP inhibitors
Specification
Ovarian cancer cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mr. Alex Sauriol

Verified customer

Submitted Jan 24 2018

Answer

Thank you for your message. I am very pleased to hear you would like to accept our offer and test ab14459 and ab14460 in IP.

ab14459
DISCOUNT CODE: ######
Expiration date: 4th Feb 2013

ab14460
DISCOUNT CODE: #######
Expiration date: 4th Feb 2013

Each code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab14459 and ab14460 in IP and include the corresponding code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreviews and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your message. i hope you enjoyed the EMBO conference.

I am sorry to confirm that to our knowledge, ab14459 and ab14460 have not been tested in IP. They have been tested in western blotting.

Therefore, I can offer a discount off a future purchase if you buy ab14459 and ab14460 now, testthem in IP and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. You would recieve one discount code for each antibody. Eachdiscount codewould be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab14459 and ab14460 in IP. I will then send a discount code. This code must be issued before purchasingthe antibodiesso please wait for my reply before ordering.

2. Purchase ab14459 and ab14460 either by phone, fax, or online (https://www.abcam.com). This purchase will be at full price.

3. Test the antibodiesin IP.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code.Each discount codecan be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab14459or ab14460 turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (HeLa cells)
Permeabilization
Yes - 0.5% Triton X100
Specification
HeLa cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Dr. Alexander Rapp

Verified customer

Submitted Apr 18 2008

Question
Answer

It is recommended to start at a 1:100 dilution. If you have any additional questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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