Overview

  • Product name

    Anti-Poly-ubiquitin (linkage-specific K48 + K48) antibody [1001C]
  • Description

    Rabbit Monoclonal [1001C] to Poly-ubiquitin (linkage-specific K48 + K48)
  • Host species

    Rabbit
  • Specificity

    ab190061 detects endogenous, Human proteins containing K48-linked polyubiquitin chains in Western blots. It detects purified, recombinant K48-linked polyubiquitin chains, but has no cross-reactivity to monoubiquitin or polyubiquitin of other linkages.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Poly-ubiquitin aa 1-76.
    Database link: P0CG47

  • Positive control

    • HepG2 cell extract, treated with 10 µM MG132 (a proteasome inhibitor).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    1001C
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml.

Target

  • Function

    Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
  • Sequence similarities

    Belongs to the ubiquitin family.
    Contains 3 ubiquitin-like domains.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • UBB antibody
    • UBB_HUMAN antibody
    • Ubiquitin antibody

Images

  • All lanes : Anti-Poly-ubiquitin (linkage-specific K48 + K48) antibody [1001C] (ab190061) at 0.5 µg/ml

    Lane 1 : Recombinant diubiquitin M1 linkage
    Lane 2 : Recombinant diubiquitin K6 linkage
    Lane 3 : Recombinant diubiquitin K11 linkage
    Lane 4 : Recombinant diubiquitin K27 linkage
    Lane 5 : Recombinant diubiquitin K29 linkage
    Lane 6 : Recombinant diubiquitin K33 linkage
    Lane 7 : Recombinant diubiquitin K48 linkage
    Lane 8 : Recombinant diubiquitin K63 linkage

    Lysates/proteins at 0.025 µg per lane.

    Secondary
    All lanes : anti-rabbit HRP (upper image); anti-mouse HRP (lower image) at 1/2000 dilution


    A single band of appropriate size was detected only in the K48-linked diubiquitin lane.

  • All lanes : Anti-Poly-ubiquitin (linkage-specific K48 + K48) antibody [1001C] (ab190061) at 0.05 µg/ml

    Lane 1 : HepG2 cell lysate, untreated
    Lane 2 : HepG2 cell lysate, treated with 10 µM MG132 (4 hours)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : anti-rabbit HRP at 1/2000 dilution


    As expected MG132 (a proteasome inhibitor) treatment resulted in the accumulation of K48-linked polyubiquitin chains attached to cellular proteins.

References

This product has been referenced in:

  • Adelöf J  et al. PA28aß overexpression enhances learning and memory of female mice without inducing 20S proteasome activity. BMC Neurosci 19:70 (2018). Read more (PubMed: 30400847) »
  • Capaz JC  et al. Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover. Front Physiol 8:344 (2017). WB . Read more (PubMed: 28603503) »
See all 2 Publications for this product

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