Anti-MTPAP antibody (ab154555)
Key features and details
- Rabbit polyclonal to MTPAP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MTPAP antibody
See all MTPAP primary antibodies -
Description
Rabbit polyclonal to MTPAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MTPAP aa 1-582.
Database link: Q9NVV4 -
Positive control
- A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 66 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA. -
Tissue specificity
Ubiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle. -
Involvement in disease
Defects in MTPAP are the cause of spastic ataxia autosomal recessive type 4 (SPAX4) [MIM:613672]. A slowly progressive neurodegenerative disease characterized by cerebellar ataxia, spastic paraparesis, dysarthria, and optic atrophy. Note=Affected individuals exhibit a drastic decrease in poly(A) tail length of representative mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105). -
Sequence similarities
Belongs to the DNA polymerase type-B-like family.
Contains 1 PAP-associated domain. -
Cellular localization
Cytoplasm. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 55149 Human
- Omim: 613669 Human
- SwissProt: Q9NVV4 Human
- Unigene: 173946 Human
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Alternative names
- 0610027A18Rik antibody
- AW551379 antibody
- EC 2.7.7.19 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154555 has been referenced in 2 publications.
- Zhang Q et al. Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency. Nucleic Acids Res 49:4689-4704 (2021). PubMed: 33836087
- Toompuu M et al. Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells. Nucleic Acids Res 46:5209-5226 (2018). PubMed: 29518244