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Signal Transduction Signaling Pathway Calcium Signaling Calcium Channels
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Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (10)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)
  • Flow Cytometry - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)

Key features and details

  • Mouse monoclonal [7e12] to Polycystin 1/PC1
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-Polycystin 1/PC1 antibody [7e12]
    See all Polycystin 1/PC1 primary antibodies
  • Description

    Mouse monoclonal [7e12] to Polycystin 1/PC1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Polycystin 1/PC1 (N terminal).

  • Epitope

    This antibody was produced to the flank-leucine rich repeat-flank region (24-180aa).
  • Positive control

    • IHC-P: Human liver, bone marrow and kidney tissue. Flow Cyt: HEK293 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     This product was previously labelled as Polycystin 1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    7e12
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Other
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-Polycystin 1/PC1 antibody [7e12] - BSA and Azide free (ab238662)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab74115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
All applications
Mouse
Rat
Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Notes
IHC-P
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      May be an ion-channel regulator. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. Involved in adhesive protein-protein and protein-carbohydrate interactions.
    • Involvement in disease

      Defects in PKD1 are the cause of polycystic kidney disease autosomal dominant type 1 (ADPKD1) [MIM:173900]. ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. Its prevalence is estimated at about 1/1000.
    • Sequence similarities

      Belongs to the polycystin family.
      Contains 1 C-type lectin domain.
      Contains 1 GPS domain.
      Contains 1 LDL-receptor class A domain.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 17 PKD domains.
      Contains 1 PLAT domain.
      Contains 1 REJ domain.
      Contains 1 WSC domain.
    • Domain

      The LDL-receptor class A domain is atypical; the potential calcium-binding site is missing.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P98161 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5310 Human
      • Entrez Gene: 18763 Mouse
      • Entrez Gene: 24650 Rat
      • Omim: 601313 Human
      • SwissProt: P98161 Human
      • SwissProt: O08852 Mouse
      • Unigene: 75813 Human
      • Unigene: 290442 Mouse
      • Unigene: 30435 Mouse
      see all
    • Alternative names

      • Autosomal dominant polycystic kidney disease 1 protein antibody
      • Autosomal dominant polycystic kidney disease protein 1 antibody
      • nPKC-D1 antibody
      • nPKC-mu antibody
      • OTTHUMP00000208669 antibody
      • OTTHUMP00000208670 antibody
      • PBP antibody
      • Pc-1 antibody
      • PKD antibody
      • Pkd1 antibody
      • PKD1_HUMAN antibody
      • Polycystic Kidney Disease 1 antibody
      • polycystic kidney disease-associated protein antibody
      • Polycystin 1 Precursor antibody
      • Polycystin-1 antibody
      • Protein kinase C mu type antibody
      • Protein kinase D antibody
      • Serine/threonine-protein kinase D1 antibody
      • transient receptor potential cation channel, subfamily P, member 1 antibody
      • TRPP1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)

      IHC image of Polycystin 1/PC1 staining in a formalin fixed, paraffin embedded normal human kidney tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)

      IHC image of Polycystin 1/PC1 staining in Human Normal Liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)

      IHC image of Polycystin 1/PC1 staining in Normal Human Bone Marrow formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Flow Cytometry - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)
      Flow Cytometry - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)

      Overlay histogram showing HEK293 cells stained with ab74115 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74115, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    Protocols

    • Mouse on Mouse staining protocol

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (10)

    Publishing research using ab74115? Please let us know so that we can cite the reference in this datasheet.

    ab74115 has been referenced in 10 publications.

    • Suwa Y  et al. Old-Age Onset Progressive Cardiac Contractile Dysfunction in a Patient with Polycystic Kidney Disease Harboring a PKD1 Frameshift Mutation. Int Heart J 60:220-225 (2019). PubMed: 30464138
    • Lu H  et al. Mutations in DZIP1L, which encodes a ciliary-transition-zone protein, cause autosomal recessive polycystic kidney disease. Nat Genet 49:1025-1034 (2017). PubMed: 28530676
    • Aguiari G  et al. Novel role for polycystin-1 in modulating cell proliferation through calcium oscillations in kidney cells. Cell Prolif 41:554-73 (2008). PubMed: 18422703
    • Xu C  et al. Human ADPKD primary cyst epithelial cells with a novel, single codon deletion in the PKD1 gene exhibit defective ciliary polycystin localization and loss of flow-induced Ca2+ signaling. Am J Physiol Renal Physiol 292:F930-45 (2007). PubMed: 17090781
    • Streets AJ  et al. Functional analysis of PKD1 transgenic lines reveals a direct role for polycystin-1 in mediating cell-cell adhesion. J Am Soc Nephrol 14:1804-15 (2003). PubMed: 12819240
    • Delmas P  et al. Constitutive activation of G-proteins by polycystin-1 is antagonized by polycystin-2. J Biol Chem 277:11276-83 (2002). PubMed: 11786542
    • Newby LJ  et al. Identification, characterization, and localization of a novel kidney polycystin-1-polycystin-2 complex. J Biol Chem 277:20763-73 (2002). PubMed: 11901144
    • Pritchard L  et al. A human PKD1 transgene generates functional polycystin-1 in mice and is associated with a cystic phenotype. Hum Mol Genet 9:2617-27 (2000). PubMed: 11063721
    • Ong AC  et al. Polycystin-1 expression in PKD1, early-onset PKD1, and TSC2/PKD1 cystic tissue. Kidney Int 56:1324-33 (1999). PubMed: 10504485
    • Ong AC  et al. Coordinate expression of the autosomal dominant polycystic kidney disease proteins, polycystin-2 and polycystin-1, in normal and cystic tissue. Am J Pathol 154:1721-9 (1999). PubMed: 10362797

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-Polycystin 1 antibody [7e12]

    Poor
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (kidney nephron)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (6)
    Loading amount
    300 µg
    Specification
    kidney nephron
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More

    Herr Dr. Andre Kraus

    Verified customer

    Submitted Mar 31 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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