Anti-Polycystin 1/PC1 antibody (ab235963)
Key features and details
- Rabbit polyclonal to Polycystin 1/PC1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Polycystin 1/PC1 antibody
See all Polycystin 1/PC1 primary antibodies -
Description
Rabbit polyclonal to Polycystin 1/PC1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Polycystin 1/PC1 aa 600-800.
Database link: P98161 -
Positive control
- IHC-P: Human colon cancer tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/500 - 1/1000.
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ICC/IF |
1/200 - 1/500.
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Notes |
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IHC-P
1/500 - 1/1000. |
ICC/IF
1/200 - 1/500. |
Target
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Function
May be an ion-channel regulator. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. Involved in adhesive protein-protein and protein-carbohydrate interactions. -
Involvement in disease
Defects in PKD1 are the cause of polycystic kidney disease autosomal dominant type 1 (ADPKD1) [MIM:173900]. ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. Its prevalence is estimated at about 1/1000. -
Sequence similarities
Belongs to the polycystin family.
Contains 1 C-type lectin domain.
Contains 1 GPS domain.
Contains 1 LDL-receptor class A domain.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 17 PKD domains.
Contains 1 PLAT domain.
Contains 1 REJ domain.
Contains 1 WSC domain. -
Domain
The LDL-receptor class A domain is atypical; the potential calcium-binding site is missing. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5310 Human
- Omim: 601313 Human
- SwissProt: P98161 Human
- Unigene: 75813 Human
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Alternative names
- Autosomal dominant polycystic kidney disease 1 protein antibody
- Autosomal dominant polycystic kidney disease protein 1 antibody
- nPKC-D1 antibody
see all
Images
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Paraffin-embedded human colon cancer tissue stained for Polycystin1/PC1 using ab235963 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Polycystin 1/PC1 (green) using ab235963 at 1/200 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab235963 has been referenced in 1 publication.
- Palander O et al. Nonredundant roles of DIAPHs in primary ciliogenesis. J Biol Chem 296:100680 (2021). PubMed: 33872598