Anti-Polycystin 2 antibody (ab244346)
Key features and details
- Rabbit polyclonal to Polycystin 2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Polycystin 2 antibody
See all Polycystin 2 primary antibodies -
Description
Rabbit polyclonal to Polycystin 2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
-
Immunogen
Recombinant fragment corresponding to Human Polycystin 2 aa 786-927.
Sequence:REDLDLDHSSLPRPMSSRSFPRSLDDSEEDDDEDSGHSSRRRGSISSGVS YEEFQVLVRRVDRMEHSIGSIVSKIDAVIVKLEIMERAKLKRREVLGRLL DGVAEDERLGRDSEIHREQMERLVREELERWESDDAASQISH
Database link: Q13563 -
Positive control
- IHC-P: Human kidney tissue. ICC/IF: BJ cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab244346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
| IHC-P | 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Functions as a calcium permeable cation channel. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. -
Tissue specificity
Strongly expressed in ovary, fetal and adult kidney, testis, and small intestine. Not detected in peripheral leukocytes. -
Involvement in disease
Defects in PKD2 are the cause of polycystic kidney disease autosomal dominant type 2 (ADPKD2) [MIM:613095]. ADPKD2 is a disorder characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. It represents approximately 15% of the cases of autosomal dominant polycystic kidney disease. ADPKD2 is clinically milder than ADPKD1 but it has a deleterious impact on overall life expectancy. -
Sequence similarities
Belongs to the polycystin family.
Contains 1 EF-hand domain. -
Domain
The C-terminal coiled-coil domain binds calcium and undergoes a calcium-induced conformation change. It is implicated in oligomerization and the interaction with PKD1. -
Cellular localization
Membrane. Endoplasmic reticulum. - Information by UniProt
-
Database links
- Entrez Gene: 530393 Cow
- Entrez Gene: 5311 Human
- Omim: 173910 Human
- SwissProt: Q4GZT3 Cow
- SwissProt: Q13563 Human
- SwissProt: O35245 Mouse
- Unigene: 181272 Human
- Unigene: 483692 Mouse
see all -
Alternative names
- APKD2 antibody
- Autosomal dominant polycystic kidney disease type II antibody
- Autosomal dominant polycystic kidney disease type II protein antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 2 antibody (ab244346)
Formalin-fixed, paraffin-embedded human kidney tissue stained for Polycystin 2 using ab244346 at 1/20 dilution in immunohistochemical analysis.
-
Immunocytochemistry/ Immunofluorescence - Anti-Polycystin 2 antibody (ab244346)PFA-fixed, Triton X-100 permeabilized BJ (human fibroblast cell line) cells stained for Polycystin 2 (green) using ab244346 at 4 µg/ml in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab244346 has not yet been referenced specifically in any publications.
