Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)

Reviews (1)Q&A (3)References (3)

Overview

  • Product name

    Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free
    See all Polyethylene glycol primary antibodies

  • Description

    Rat monoclonal [26A04] to Polyethylene glycol - BSA and Azide free

  • Host species

    Rat

  • Specificity

    ab94764 recognizes linear 20 kDa PEG, linear 5 kDa PEG, linear 600 Da PEG and 10 kDa branched PEG. It also binds to BSA conjugated PEG by western blot.

  • Tested applications

    Suitable for: IHC-P, WB, Indirect ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    5 kDa PEG conjugated to KLH by a MBS linker.

  • Positive control

    • PEGylated protein

  • General notes

    Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents
    when performing ELISA using anti-PEG products.

    Learn more about PEG RabMAb primary antibodies and kit - PEG RabMAb Product Portal Page

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    Constituent: Tris buffered saline

  • Carrier free

    Yes
  • Concentration information loading...

  • Purification notes

    Ceramic hydroxyapatite (CHT) chromatography from in vitro production.

  • Clonality

    Monoclonal

  • Clone number

    26A04

  • Isotype

    IgM

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 8 µg/ml.
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.
Indirect ELISA Use a concentration of 2 µg/ml.

Target

  • Alternative names

    • PEG antibody

Images

  • Indirect ELISA - Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)
    Indirect ELISA - Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)
    Indirect ELISA analysis of a PEG panel using ab94764.
  • Western blot - Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)
    Western blot - Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)
    Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764) + PEGylated protein

    Predicted band size: 1 kDa

References

Publishing research using ab94764? Please let us know so that we can cite the reference in this datasheet.

ab94764 has been referenced in 3 publications.

  • Burtea C  et al. Screening for peptides targeted to IL-7Ra for molecular imaging of rheumatoid arthritis synovium. Arthritis Res Ther 18:230 (2016). PubMed: 27729062
  • Clark AJ  et al. CRLX101 nanoparticles localize in human tumors and not in adjacent, nonneoplastic tissue after intravenous dosing. Proc Natl Acad Sci U S A 113:3850-4 (2016). PubMed: 27001839
  • Williams RM  et al. Mesoscale nanoparticles selectively target the renal proximal tubule epithelium. Nano Lett 15:2358-64 (2015). PubMed: 25811353

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

Which citrate antigen retrieval buffer does not contain Tween detergent?

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Answer

To avoid Tween20 and other PEG-containing detergents, I recommend antigen retrieval buffers https://www.abcam.com/100x-citrate-buffer-ph-60-ab64236.html (100x Citrate Buffer pH 6.0, 50mL) or https://www.abcam.com/10x-citrate-buffer-ph-60-ab64214.html (10x Citrate Buffer pH 6.0, 125mL). Other citrate antigen retrieval buffers such as ab94674 and ab93678 do contain detergent that may interfere with detection of PEG by antibody ab94764.

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Question

Is the antibody effective againist Methoxy-PEG (PEG-OMe) or standard PEG (PEG-OH). Moreover I want to know if you have idea of the affinity for short PEG chains (below 600Da).
For my application (immobilization of pegyated molecules on surfaces) it would be necessary to bind short PEG chains (200-300Da) even with low affinity.

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Answer

I can confirm that we have determined that the specificity of the antibody (ab94764: Anti-Polyethylene glycol antibody) is to the linear backbone of PEG and that it will not recognize the methoxy group.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Polyethylene glycol antibody [26A04]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Atheroma on aorta of ApoE-KO mice)
Specification
Atheroma on aorta of ApoE-KO mice
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - baths in 0.1% Tween-20 diluted in PBS
Blocking step
Protein-Free Blocking Buffer, Pierce, Cat N° 37570 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
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Abcam user community

Verified customer

Submitted Jan 12 2012

Question

Could you please explain me how this would change my results when this antibody is detected with an anti-rat IgG?

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Answer

Thank you for your reply. If the secondary antibody is directed against the heavy and light chains (H+L) of rat IgG, you will still be able to observe specific signal because the light chains are conserved between isotypes. If the secondary antibody is specific for a certain gamma heavy chain type (IgG1, 2, etc), then you will not observe specific signal.

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