Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)
![Indirect ELISA - Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)](https://www.abcam.com/ps/products/94/ab94764/Images/ab94764-109285-anti-polyethylene-glycol-antibody-26a04-indirect-elisa.jpg "Indirect ELISA - Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)")
Key features and details
- Rat monoclonal [26A04] to Polyethylene glycol - BSA and Azide free
- Suitable for: WB, Indirect ELISA
- Reacts with: Species independent
- Isotype: IgM
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free
See all Polyethylene glycol primary antibodies -
Description
Rat monoclonal [26A04] to Polyethylene glycol - BSA and Azide free -
Host species
Rat -
Specificity
ab94764 recognizes linear 20 kDa PEG, linear 5 kDa PEG, linear 600 Da PEG and 10 kDa branched PEG. It also binds to BSA conjugated PEG by western blot. -
Tested applications
Suitable for: WB, Indirect ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
5 kDa PEG conjugated to KLH by a MBS linker.
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Positive control
- PEGylated protein
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General notes
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents
when performing ELISA using anti-PEG products.Learn more about PEG RabMAb primary antibodies and kit - PEG RabMAb Product Portal Page
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Tris buffered saline -
Carrier free
Yes -
Concentration information loading... -
Purification notes
Ceramic hydroxyapatite (CHT) chromatography from in vitro production. -
Clonality
Monoclonal -
Clone number
26A04 -
Isotype
IgM -
Research areas
Associated products
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 1 kDa.
|
|
| Indirect ELISA |
Use a concentration of 2 µg/ml.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 1 kDa. |
|
Indirect ELISA
Use a concentration of 2 µg/ml. |
Target
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Alternative names
- PEG antibody
Images
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Indirect ELISA - Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)Indirect ELISA analysis of a PEG panel using ab94764.
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![Western blot - Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)](https://www.abcam.com/ps/products/94/ab94764/Images/ab94764-109284-anti-polyethylene-glycol-antibody-26a04-western-blot.jpg)
Western blot - Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764)Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764) + PEGylated protein
Predicted band size: 1 kDa
Datasheets and documents
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Datasheet download
References (6)
ab94764 has been referenced in 6 publications.
- Han SJ et al. Renal proximal tubular NEMO plays a critical role in ischemic acute kidney injury. JCI Insight 5:N/A (2020). PubMed: 32941183
- Han SJ et al. Selective nanoparticle-mediated targeting of renal tubular Toll-like receptor 9 attenuates ischemic acute kidney injury. Kidney Int 98:76-87 (2020). PubMed: 32386967
- Karabasz A et al. In vivo Studies on Pharmacokinetics, Toxicity and Immunogenicity of Polyelectrolyte Nanocapsules Functionalized with Two Different Polymers: Poly-L-Glutamic Acid or PEG. Int J Nanomedicine 14:9587-9602 (2019). PubMed: 31824153
- Burtea C et al. Screening for peptides targeted to IL-7Ra for molecular imaging of rheumatoid arthritis synovium. Arthritis Res Ther 18:230 (2016). PubMed: 27729062
- Clark AJ et al. CRLX101 nanoparticles localize in human tumors and not in adjacent, nonneoplastic tissue after intravenous dosing. Proc Natl Acad Sci U S A 113:3850-4 (2016). PubMed: 27001839
- Williams RM et al. Mesoscale nanoparticles selectively target the renal proximal tubule epithelium. Nano Lett 15:2358-64 (2015). PubMed: 25811353
