Recombinant
RabMAb

Recombinant Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Overview

  • Product name

    Anti-Polyethylene glycol antibody [EPR21993-154]
    See all Polyethylene glycol primary antibodies
  • Description

    Rabbit monoclonal [EPR21993-154] to Polyethylene glycol
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Polyethylene glycol.

  • Positive control

    • WB: BSA-methoxy PEG 5K lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21993-154
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ELISA 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Alternative names

      • PEG antibody

    Images

    • Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857) at 1/1000 dilution + BSA-methoxy PEG 5K, 10 ng

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 250 kDa
      why is the actual band size different from the predicted?



      Exposure time: 92 seconds.

      Blocking/Diluting buffer: 5% NFDM/TBST.

    • Direct ELISA antigen dose-response curve using AffniPure Goat Anti-Mouse IgG (H&L) at 1 µg/ml concentration. The detector antibody was ab203857 anti-Polyethylene glycol at 1 µg/ml. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

      Antigen concentration range: 0-1000 ng/ml.

      Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

      Washing Buffer: 1 X TBST.

      Substrate: p-nitrophenyl phosphate (PNPP).

      Coloration time: 10 min.

    • Direct ELISA antigen dose-response curve using ab203857 at 2 µg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

      Antigen concentration range: 0-1000 ng/ml.

      Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

      Washing Buffer: 1 X TBST.

      Substrate: 3,3',5,5'-Tetramethylbenzidine (TMB).

      Coloration time: 2 min.

    • Direct ELISA antigen dose-response curve using ab203857 at 2 µg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

      Antigen concentration range: 0-1000 ng/ml.

      Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

      Washing Buffer: 1 X TBST.

      Substrate: 3,3',5,5'-Tetramethylbenzidine (TMB).

      Coloration time: 2 min.

    • Direct ELISA antigen dose-response curve using ab203857 at 0.05 µg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

      Antigen concentration range: 0-0.05µg/ml.

      Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

      Washing Buffer: 1 X TBST.

      Substrate: p-nitrophenyl phosphate (PNPP).

      Coloration time: 15 min.

    • Direct ELISA antigen dose-response curve using ab203857 at 0.05 µg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

      Antigen concentration range: 0-0.05µg/ml.

      Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

      Washing Buffer: 1 X TBST.

      Substrate: p-nitrophenyl phosphate (PNPP).

      Coloration time: 15 min.

    • Method: APS sensor+ Antigen (methoxy PEG800 at 0.625 µg/ml) + Blocking (BSA) + Antibody (ab203857 at 2, 1, 0.5, 0.25, 0.125µg/ml).

      Results:

      KD (M) = 5.81E-11

      kon(1/Ms) = 2.70E+05

      kon Error = 6.28E+02

      kdis(1/s) = 1.57E-05

      kdis Error = 4.49E-07

      Full X2= 10.143

      Full R2= 0.9993

      Testing Biosensor: Aminopropylsilane (APS) Biosensors

      Testing machine: Fortebio RED96e

    • Method: APS sensor+ Antigen (methoxy PEG800 at 25 µg/ml) + Blocking (BSA) + Antibody (ab203857 at 0.5, 0.25, 0.125, 0.0625, 0.03125µg/ml).

      Results:

      KD (M) = 1.29E-10

      kon(1/Ms) = 2.23E+06

      kon Error = 1.45E+04

      kdis(1/s) = 2.88E-04

      kdis Error = 1.74E-06

      Full X2 = 3.475

      Full R2 = 0.9865

      Testing Biosensor: Aminopropylsilane (APS) Biosensors

      Testing machine: Fortebio RED96e

    References

    ab203857 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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