Recombinant Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (ab275332)
![Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)](https://www.abcam.com/ps/products/275/ab275332/Images/ab275332-403583-AntiPolyethylene-glycol-antibody-EPR21993154-western-blot-bsamethoxy-peg.jpg "Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [EPR21993-154] to Polyethylene glycol - Mouse IgG2b
- Suitable for: WB, ELISA
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b
See all Polyethylene glycol primary antibodies -
Description
Mouse monoclonal [EPR21993-154] to Polyethylene glycol - Mouse IgG2b -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: BSA-methoxy PEG 5K.
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General notes
This mouse antibody has been engineered from a RabMAb parent antibody ab203857. By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21993-154 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000.
|
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| ELISA |
Use at an assay dependent concentration.
Use at 50ng/ml. |
| Notes |
|---|
|
WB
1/5000. |
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ELISA
Use at an assay dependent concentration. Use at 50ng/ml. |
Target
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Alternative names
- PEG antibody
Images
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Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)Lane 1 : Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (ab275332) at 1/5000 dilution
Lane 2 : Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857) at 1/5000 dilution
All lanes : BSA-methoxy PEG 5K, 10 ng
Secondary
Lane 1 : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)](https://www.abcam.com/ps/products/275/ab275332/Images/ab275332-403584-AntiPolyethylene-glycol-antibody-EPR21993154-elisa.jpg)
ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - Chimeric (ab275332)ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab275332, 50 ng/ml).
Antigen used at 0-500ng: NH2-PEG 5K-OCH3, NH2-PEG20K-NH2, PEG1000, PEG1000-BSA, mPEG800, mPEG800-BSA.
Secondary antibody used at 1/1000: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H&L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab275332 has not yet been referenced specifically in any publications.