Overview

  • Product name

    Anti-Polyethylene glycol antibody [PEG-2-128] - BSA and Azide free
    See all Polyethylene glycol primary antibodies
  • Description

    Rabbit monoclonal [PEG-2-128] to Polyethylene glycol - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Polyethylene glycol conjugated to keyhole limpet haemocyanin. Synthetic peptide corresponds to Polyethylene glycol 20K (PEG20K).

  • Epitope

    This antibody recognizes the backbone of the Polyethylene glycol molecule.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab133471 is useful in measuring the pharmacokinetics of Polyethylene glycol modified molecules. This antibody is specific to the backbone of PEG molecule.  
    For detection of PEG methoxy group, see Anti-Polyethylene glycol antibody [PEG-B-47] - ab51257

    For PEG specific ELISA kit - see Polyethylene Glycol ELISA Kit - ab215546

    Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents.
    Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents
    when performing ELISA using anti-PEG products.

    Antibody Isotype:  Please note, this RabMAb product's isotype is Rabbit IgM.  Anti-IgM secondary is recommended as a secondary antibody.
    List of anti-Rabbit IgM secondaries.

    Learn more about PEG RabMAb primary antibodies and kit - PEG RabMAb Product Portal Page

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This RabMAb product's isotype is Rabbit IgM. Therefore, KD values were provided by loading the antigen first before testing the antibody.
    When compared to loading the antibody first and then the antigen, this method can give approximately 10-1 difference as determined by using ab51257 - Anti-Polyethylene glycol antibody [PEG-B-47] (of rabbit IgG isotype) as a control:

    Antigen loaded first: 1.51E-10.

    Antibody loaded first: 2.41E-09

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
  • Dissociation constant (KD)

    KD = 8.24 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    PEG-2-128
  • Isotype

    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

The MW of the WB band for ab215363 varies based on the PEGylated molecule detected.

ELISA Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.

Target

  • Alternative names

    • PEG antibody

Images

  • Direct ELISA antigen dose-response curve using purified ab133471. Antigen concentration of 0-100 ng/mL. A HRP-conjugated goat anti-rabbit IgM (1/1000) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133471).

  • Direct ELISA antigen dose-response curve using purified ab133471. Antigen concentration of 0-10000 ng/mL. A HRP-conjugated goat anti-rabbit IgM (1/1000) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133471).

  • Sandwich ELISA antigen dose-response curve using purified ab133471.

    Capture antibody - ab133471 (10000 ng/ml).

    Antigens - MBP-PEG20K and ADI-PEG (0-1000 ng/ml).

    Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133471).

  • This antibody affinity data was generated using the same anti-PEG antibody clone, PEG-2-128, in a different buffer formulation (ab133471).

    Method:Protein A sensor + Antigen (PEG 5K-BSA at 0.3µg/ml)+ antibody (ab133471 at  0.0312, 0.0625, 0.125, 0.25, 0.5, 1 µg/m).

    Results:

    Sample ID: PEG-5K-BSA

    KD (M): 2.41E-09

    kon(1/Ms): 2.67E+04

    kon Error: 6.81E+02

    kdis(1/s): 6.43E-05

    kdis Error: 2.92E-06

    Full X^2: 0.018444

    Full R^2: 0.982059

  • Various concentrations of Polyethylene glycol polymers with amine end groups were coated in 96 wells. 2 ug/ml of unpurified ab133471 was added. After washing, samples were incubated with 1 ug/ml of HRP-conjugated anti-rabbit IgM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133471).

  • Various concentrations of Polyethylene glycol 20K (PEG20K) conjugated MBP and branched Polyethylene glycol conjugated BSA were coated in 96 wells. 2 ug/ml of unpurified ab133471 was added. After washing, samples were incubated with 1 ug/ml of HRP-conjugated anti-rabbit IgM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133471).

  • This ELISA data was generated using the same anti-PEG antibody clone, PEG-2-128, in a different buffer formulation (cat# ab133471).

    10 ug/ml of unpurified ab133471 was coated into 96 wells. Serial dilution of Polyethylene glycol 20K (PEG20K) conjugated MBP and PEGylated ADI were added. After washing, 1 ug/ml of biotinylated anti-Polyethylene glycol antibody was added. Streptavidin-HRP was then used to develop the color.

References

This product has been referenced in:

See all 2 Publications for this product

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