Recombinant
RabMAb

Recombinant Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)

Overview

  • Product name

    Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free
    See all Polyethylene glycol primary antibodies
  • Description

    Rabbit monoclonal [PEG-B-47] to Polyethylene glycol - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Polyethylene glycol conjugated to keyhole limpet haemocyanin. KLH-Polyethylene glycol with a terminal methoxy group.

  • Positive control

    • IHC-P: Mouse kidney, spleen, muscle and liver tissue - (animals injected with a PEGylated protein).
  • General notes

    ab170969 is the carrier-free version of ab51257 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab170969 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 2.41 x 10 -9 M
    Learn more about KD
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    PEG-B-47
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

This antibody only works on the tissues when the animals are injected with a PEGylated protein.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Alternative names

    • PEG antibody

Images

  • This IHC data was generated using the same anti-PEG antibody clone, PEG-B-47, in a different buffer formulation (cat# ab51257).

    ab51257, unpurified,  staining mouse kidney at 1:10 dilution. Left panel: without animal injection with a PEGylated protein. Right panel: with animal injection with a PEGylated protein.

  • ELISA assay using ab51257 to detect different forms of PEG. PEG40000-BSA is a 40 kDa PEG molecule attached to BSA. PEG5000-BSA is a 5 kDa linear PEG molecule attached to BSA.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • Sandwich ELISA-Left Graph generated using unpurified ab51257 and right using purified ab51257 at 1μg/mL. Antigen concentration range 0.0156 - 1 μg/mL for PEG-IFN-α-2b. Secondary antibody was an Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG(H+L) at 1/2500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • Direct ELISA-Left Graph generated using unpurified ab51257 and right using purified ab51257 at 1μg/mL. Antigen concentration range 0.0039 - 0.25μg/mL for PEG-BSA. Secondary antibody was an Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG(H+L) at 1/2500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • This antibody affinity data was generated using the same anti-PEG antibody clone, PEG-B-47, in a different buffer formulation (ab51257).

    Method:Protein A sensor + Antigen (PEG 5K-BSA at 0.3µg/ml)+ antibody (ab51257 at  0.0312, 0.0625, 0.125, 0.25, 0.5, 1 µg/m).

    Results:

    Sample ID: PEG-5K-BSA

    KD (M): 2.41E-09

    kon(1/Ms): 2.67E+04

    kon Error: 6.81E+02

    kdis(1/s): 6.43E-05

    kdis Error: 2.92E-06

    Full X^2: 0.018444

    Full R^2: 0.982059

  • Direct ELISA-Left Graph generated using unpurified ab51257 and right using purified ab51257 at 1μg/mL. Antigen concentration range 0.0156 - 1 μg/mL for ADI-PEG. Secondary antibody was an Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG(H+L) at 1/2500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • Direct ELISA-Left Graph generated using unpurified ab51257 and right using purified ab51257 at 1μg/mL. Antigen concentration range 0.0078 - 0.5 μg/mL for both PEG5000 and PEG20000. Secondary antibody was an Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG(H+L) at 1/2500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • ab51257, unpurified, staining mouse spleen at 1:10 dilution. Left panel: without animal injection with a PEGylated protein. Right panel: with animal injection with a PEGylated protein.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • ab51257, unpurified, staining mouse muscle at 1:10 dilution. Left panel: without animal injection with a PEGylated protein. Right panel: with animal injection with a PEGylated protein.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • ab51257, unpurified,  staining mouse liver at 1:10 dilution. Left panel: without animal injection with a PEGylated protein. Right panel: with animal injection with a PEGylated protein.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • Comparison of ab51257 and Vendor A mouse MAb in Direct ELISA assy.

    Goat anti-rabbit IgG-AP used for anti-PEG-47 detection; goat anti-mouse IgM-AP used for Vendor A MAb detection.

    Fig 1a. Direct ELISA using 1 ug/mL of PEG-GCSF.

    Fig 1b. Direct ELISA using 1 ug/mL of PEG-IFN.

    Comparison to other anti-PEG: In both direct and sandwich ELISA assays, ab51257 shows greater affinity and accuracy than other anti-PEG antibodies when determining the concentration of PEG or PEG-modified proteins. Results were similar whether detecting PEG itself or PEG-modified targets.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • Comparison of sandwich ELISA using RabMAb/RabMAb (ab51257/ab51257) and MAb/RabMAb (Vendor A/ab51257) for capture/detection.

    Ab51257/ab51257*: Plate coated with 5 ug/mL of #47; 5 ug/mL of #47 used for detection (*Anti-PEG 47 biotin labeled)

    Vendor A/ ab51257*: Plates coated with 100 ug/mL of Vendor A Mouse MAb; 5 ug/mL of #47 used for detection (*Anti-PEG 47 biotin labeled)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

  • Comparison of 10 ug/well of activated linear (PEG5K) and branched (PEG40K) PEG using 5 ug/ml of ab51257 in Direct ELISA assay.

    Accuracy: By detecting the methoxy group of the PEG molecule itself, ab51257 is useful in measuring the pharmacokinetics of PEG-modified molecules in vivo. Data indicate that ab51257 detects various length Y-chain PEG molecules as well as single chain PEG molecules with equal affinity. Ab51257 does not cross react with non-specific targets in blood or serum.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).

References

ab170969 has not yet been referenced specifically in any publications.

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