Recombinant Anti-Polyethylene glycol antibody [RM105] (ab190652)
![Western blot - Anti-Polyethylene glycol antibody [RM105] (ab190652)](https://www.abcam.com/ps/products/190/ab190652/Images/ab190652-226132-anti-polyethylene-glycol-antibody-rm105-western-blot.jpg "Western blot - Anti-Polyethylene glycol antibody [RM105] (ab190652)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM105] to Polyethylene glycol
- Suitable for: ICC, WB, ELISA, IHC-P, Flow Cyt, IP
Overview
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Product name
Anti-Polyethylene glycol antibody [RM105]
See all Polyethylene glycol primary antibodies -
Description
Rabbit monoclonal [RM105] to Polyethylene glycol -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, ELISA, IHC-P, Flow Cyt, IPmore details -
Immunogen
Chemical/ Small Molecule corresponding to Polyethylene glycol conjugated to Keyhole Limpet Haemocyanin (KLH). (KLH-PEG with terminal methoxy group).
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Positive control
- Mouse liver and kidney tissue injected with PEG-BSA; PEGylated BSA.
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General notes
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody (RM105) reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody. This detergent can safely be used in ELISA assays.
Learn more about PEG RabMAb products and kit - PEG RabMAb Product Portal Page
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab190652 was purified from an animal origin–free and protein-free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM105 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 0.05 - 1 µg/ml.
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| ELISA |
Use a concentration of 0.01 - 0.3 mg/ml.
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| IHC-P |
Use a concentration of 0.5 - 2 µg/ml.
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| Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC
Use at an assay dependent concentration. |
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WB
Use a concentration of 0.05 - 1 µg/ml. |
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ELISA
Use a concentration of 0.01 - 0.3 mg/ml. |
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IHC-P
Use a concentration of 0.5 - 2 µg/ml. |
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Flow Cyt
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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IP
Use at an assay dependent concentration. |
Target
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Alternative names
- PEG antibody
Images
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Western blot - Anti-Polyethylene glycol antibody [RM105] (ab190652)All lanes : Anti-Polyethylene glycol antibody [RM105] (ab190652) at 0.1 µg/ml
Lane 1 : BSA at 0.2 µg
Lane 2 : PEGylated BSA at 0.2 µg
Lane 3 : BSA at 0.1 µg
Lane 4 : PEGylated BSA at 0.1 µg
Lane 5 : BSA at 0.05 µg
Lane 6 : PEGylated BSA at 0.05 µg
Lane 7 : BSA at 0.02 µg
Lane 8 : PEGylated BSA at 0.02 µg
Lane 9 : BSA at 0.01 µg
Lane 10 : PEGylated BSA at 0.01 µg -
![ELISA - Anti-Polyethylene glycol antibody [RM105] (ab190652)](https://www.abcam.com/ps/products/190/ab190652/Images/ab190652-226135-anti-polyethylene-glycol-antibody-rm105-elisa.jpg)
ELISA - Anti-Polyethylene glycol antibody [RM105] (ab190652)ELISA of three different PEGylated BSA using ab190652 at 0.3 µg/ml, following AP conjugated goat anti-rabbit IgG.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [RM105] (ab190652)](https://www.abcam.com/ps/products/190/ab190652/Images/ab190652-226134-anti-polyethylene-glycol-antibody-rm105-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [RM105] (ab190652)Immunohistochemical analysis of Mouse liver and kidney tissue labeling PEG with ab190652 at 0.5 µg/ml. Mouse had been injected with PEG-BSA (left panels) or BSA (right panels) for 3 hours before sampling.
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![Anti-Polyethylene glycol antibody [RM105] (ab190652)](https://www.abcam.com/ps/products/190/ab190652/Images/ab190652-4-benefits-of-recombinant-antibodies.png)
Anti-Polyethylene glycol antibody [RM105] (ab190652)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab190652 has been referenced in 1 publication.
- Geeurickx E et al. The generation and use of recombinant extracellular vesicles as biological reference material. Nat Commun 10:3288 (2019). PubMed: 31337761