Product namePolyethylene Glycol ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 4 3% Inter-assay Sample n Mean SD CV% Overall 4 6%
Sample typeSerum, Plasma, Cell culture media
Range2 ng/ml - 200 ng/ml
Sample specific recovery Sample type Average % Range Serum 91 82% - 101% Cell culture media 104 99% - 108% Heparin Plasma 70 64% - 77% EDTA Plasma 84 77% - 90% Citrate Plasma 79 75% - 85%
Assay durationMultiple steps standard assay
Polyethylene Glycol ELISA Kit (ab138914) has been re-developed. Specifcally, the way we manufacture some of the kit’s components has changed (wash buffer, TMB and stop solution). This new kit has the same name but a different product number (ab215546), and the performance of the new kit is the same.
Polyethylene Glycol (PEG) RabMAb® in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate competitive quantitative measurement of PEGylated molecules in plasma, serum and cell culture media.
The Polyethylene Glycol (PEG) RabMAb® ELISA Kit operates on the basis of competition between enzyme HRP conjugated PEG and PEG labeled molecules for a limited number of binding sites on the surface of 96-wells coated with anti-PEG RabMab® antibody. The extent of color development resulting from interaction between HRP and the substrate TMB is inversely proportional to the amount of PEGylated molecules in the sample. For example, the absence of PEGylated molecules in the sample will result in a bright blue color, whereas the presence of PEGylated molecules will result in decreased or no color development.
This kit is developed to determine levels of PEGylated molecules in samples such as serum, plasma or cell culture medium via ELISA.
Users must PEGylate their interested molecules. For pharmaco kinetic experiments, the PEGylated molecules will be used to construct standard curves.
The use of this kit requires the end user to have at least 250 ng of PEGylated compound of interest to use as reference standard. The included PEG-BSA is a reference sample only.
Polyethylene glycol (PEG) is an O-CH2-CH2 polymer, which is water-soluble, nontoxic, non-antigenic, and biocompatible. Covalent conjugation of PEG to therapeutic proteins increases the in vivo stability by protecting the protein from degradation, masking its immunogenic sites and reducing clearance. Typically, PEGylation uses nonspecific reactions with nucleophilic residues and produces mixtures of PEGylated positional isomers. Qualitative and quantitative analysis of PEGylated molecules is important for both drug development and clinical application.
Tested applicationsSuitable for: Competitive ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antigen/Antibody Diluent Buffer 1X 1 x 20ml Anti-PEG coated microplate (12 x 8 well strips) 1 unit PEG-conjugated HRP 60X 1 x 150µl Plate Seals 1 unit Reference Standard (PEG-BSA) 1 x 200ng Stop Solution 1 x 12ml TMB Substrate 1 x 12ml
Our Abpromise guarantee covers the use of ab215546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
ab215546 has not yet been referenced specifically in any publications.