Overview

  • Product name

    Polyethylene Glycol ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4 3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 4 6%
  • Sample type

    Serum, Plasma, Cell culture media
  • Assay type

    Competitive
  • Range

    2 ng/ml - 200 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 91 82% - 101%
    Cell culture media 104 99% - 108%
    Hep Plasma 70 64% - 77%
    EDTA Plasma 84 77% - 90%
    Cit plasma 79 75% - 85%

  • Assay duration

    Multiple steps standard assay
  • Product overview

    Polyethylene Glycol (PEG) RabMAb® in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate competitive quantitative measurement of PEGylated molecules in plasma, serum and cell culture media.


    The Polyethylene Glycol (PEG) RabMAb® ELISA Kit operates on the basis of competition between enzyme HRP conjugated PEG and PEG labeled molecules for a limited number of binding sites on the surface of 96-wells coated with anti-PEG RabMab® antibody. The extent of color development resulting from interaction between HRP and the substrate TMB is inversely proportional to the amount of PEGylated molecules in the sample. For example, the absence of PEGylated molecules in the sample will result in a bright blue color, whereas the presence of PEGylated molecules will result in decreased or no color development.


    This kit is developed to determine levels of PEGylated molecules in samples such as serum, plasma or cell culture medium via ELISA.


    Users must PEGylate their interested molecules. For pharmaco kinetic experiments, the PEGylated molecules will be used to construct standard curves.


    The use of this kit requires the end user to have at least 250 ng of PEGylated compound of interest to use as reference standard. The included PEG-BSA is a reference sample only.


    This kit does not contain Tween 20. 


    Consult our PEG FAQs page for more information:https://www.abcam.com/primary-antibodies/polyethylene-glycol-peg-faqs


    Polyethylene Glycol ELISA Kit (ab138914) has been re-developed.  Specifcally, the way we manufacture some of the kit’s components has changed (wash buffer, TMB and stop solution). This new kit has the same name but a different product number (ab215546), and the performance of the new kit is the same.

  • Notes

    Polyethylene glycol (PEG) is an O-CH2-CH2 polymer, which is water-soluble, nontoxic, non-antigenic, and biocompatible. Covalent conjugation of PEG to therapeutic proteins increases the in vivo stability by protecting the protein from degradation, masking its immunogenic sites and reducing clearance. Typically, PEGylation uses nonspecific reactions with nucleophilic residues and produces mixtures of PEGylated positional isomers. Qualitative and quantitative analysis of PEGylated molecules is important for both drug development and clinical application.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antigen/Antibody Diluent Buffer 1X 1 x 20ml
    Anti-PEG coated microplate (12 x 8 well strips) 1 unit
    PEG-conjugated HRP 60X 1 x 150µl
    Plate Seals 1 unit
    Reference Standard (PEG-BSA) 1 x 200ng
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Alternative names

    • PEG

Applications

Our Abpromise guarantee covers the use of ab215546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab215546 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215546.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up