Overview

  • Product name

    Anti-polyHistidine antibody - Azide free
    See all polyHistidine primary antibodies
  • Description

    Rabbit polyclonal to polyHistidine - Azide free
  • Host species

    Rabbit
  • Specificity

    Reacts specifically with polyhistidine tagged proteins
  • Tested applications

    Suitable for: WB, ELISA, Dot blotmore details
  • Species reactivity


    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic poly-histidine peptide Tag.

Properties

Applications

Our Abpromise guarantee covers the use of ab58992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000.
ELISA 1/2000 - 1/10000.
Dot blot 1/500 - 1/2000.

Target

  • Relevance

    Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide 'affinity handles' (tags) designed to enable the selective identification of the protein of interest. These sequences of tails or tags are genetically engineered away from the protein active site, by insertion at the N or C terminus. It has been reported that the addition of a consecutive Histidine amino acid residue tail creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the Histidine tagged product.

References

ab58992 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



La concentration en anticorps d'un antisérum est estimé entre 1 et 10 mg/ml.
Le tableau de la page web suivante peut, je pense, également vous être utile : https://www.abcam.com/index.html?pageconfig=technicalfaqs#8.

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