Overview

  • Product name

    Anti-polyHistidine antibody [HIS-1] (HRP)
    See all polyHistidine primary antibodies
  • Description

    Mouse monoclonal [HIS-1] to polyHistidine (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: ELISA, Dot blot, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant polyHistidine tagged fusion protein.

  • Positive control

    • Bacteria lysates expressing a recombinant Histidine tagged fusion protein.
  • General notes

    Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.

    The conjugate was prepared by conjugation of HRP to Mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma. Molar ratio Ab/Enzyme: 0.8 to 1.5 Enzyme activity: at least 400 U/ml

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Purified Immunoglobulin
  • Primary antibody notes

    Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.
  • Clonality

    Monoclonal
  • Clone number

    HIS-1
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Dot blot 1/2000.
WB 1/2000.

Target

  • Relevance

    Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide 'affinity handles' (tags) designed to enable the selective identification of the protein of interest. These sequences of tails or tags are genetically engineered away from the protein active site, by insertion at the N or C terminus. It has been reported that the addition of a consecutive Histidine amino acid residue tail creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the Histidine tagged product.

References

This product has been referenced in:

  • Kim YJ  et al. Molecular and Structural Characterization of the Tegumental 20.6-kDa Protein in Clonorchis sinensis as a Potential Druggable Target. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28273846) »
See 1 Publication for this product

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