Anti-Polymeric immunoglobulin receptor / PIGR antibody [7C1] (ab170321)

Overview

  • Product name
    Anti-Polymeric immunoglobulin receptor / PIGR antibody [7C1]
    See all Polymeric immunoglobulin receptor / PIGR primary antibodies
  • Description
    Rat monoclonal [7C1] to Polymeric immunoglobulin receptor / PIGR
  • Host species
    Rat
  • Tested applications
    Suitable for: IHC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Mouse Polymeric immunoglobulin receptor/ PIGR.
    Database link: P01833

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: 99.9% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    7C1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa.

Target

  • Function
    This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.
  • Sequence similarities
    Contains 5 Ig-like V-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    N-glycosylation is not necessary for Ig binding.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hepatocellular carcinoma associated protein TB6 antibody
    • Hepatocellular carcinoma-associated protein TB6 antibody
    • MGC125361 antibody
    • MGC125362 antibody
    • Phosphatidylinositol glycan, class R antibody
    • PIGR antibody
    • PIGR_HUMAN antibody
    • Poly Ig receptor antibody
    • Poly-Ig receptor antibody
    • Polymeric immunoglobulin receptor antibody
    • Secretory component antibody
    • Transmembrane secretory component antibody
    see all

References

ab170321 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (Thymic epithelial cells)
Permeabilization
No
Gating Strategy
CD45- EpCAM+ MHCII lo Ly6d hi - gating on post Aire TECs.
Specification
Thymic epithelial cells
Username

Abcam user community

Verified customer

Submitted Feb 28 2018

Application
ELISA
Sample
Human Purified protein (secretory IgA and IgA)
Specification
secretory IgA and IgA
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type
Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted Jun 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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