Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)

Overview

  • Product name

    Anti-Polymeric immunoglobulin receptor/PIGR antibody
    See all Polymeric immunoglobulin receptor/PIGR primary antibodies
  • Description

    Rabbit polyclonal to Polymeric immunoglobulin receptor/PIGR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Polymeric immunoglobulin receptor/PIGR aa 180-429.

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Molt4 and Raji cells; Cal27 xenograft tissue.
  • General notes

    Previously labelled as PIGR. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/300. Predicted molecular weight: 83 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function

    This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.
  • Sequence similarities

    Contains 5 Ig-like V-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    N-glycosylation is not necessary for Ig binding.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Hepatocellular carcinoma associated protein TB6 antibody
    • Hepatocellular carcinoma-associated protein TB6 antibody
    • MGC125361 antibody
    • MGC125362 antibody
    • Phosphatidylinositol glycan, class R antibody
    • PIGR antibody
    • PIGR_HUMAN antibody
    • Poly Ig receptor antibody
    • Poly-Ig receptor antibody
    • Polymeric immunoglobulin receptor antibody
    • Secretory component antibody
    • Transmembrane secretory component antibody
    see all

Images

  • Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg

    Predicted band size: 83 kDa

  • ab96196 at 1/200 dilution staining Polymeric immunoglobulin receptor/PIGR in methanol fixed HepG2 cells. Lower image is co-stained with Hoechst 33342.

  • ab96196 at 1/500 dilution staining Polymeric immunoglobulin receptor/PIGR in paraffin-embedded Cal27 xenograft tissue.

References

This product has been referenced in:

  • Deleon-Pennell KY  et al. Glycoproteomic Profiling Provides Candidate Myocardial Infarction Predictors of Later Progression to Heart Failure. ACS Omega 4:1272-1280 (2019). Read more (PubMed: 30729226) »
  • Zhou M  et al. Expression of polymeric immunoglobulin receptor and its biological function in endometrial adenocarcinoma. J Cancer Res Ther 15:420-425 (2019). Read more (PubMed: 30964121) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mice Hepatocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
35 µg
Specification
Mice Hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 15 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat Hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat Hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Dec 21 2016

Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (6% SDS-PAGE)
Loading amount
15 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 17 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (WIF-B cells, the hepatoma-derived hybrid cell line)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
35 µg
Specification
WIF-B cells, the hepatoma-derived hybrid cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 04 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (WIF-B cells, the hepatoma-derived hybrid cell line)
Permeabilization
Yes - 0.2% Triton X-100
Specification
WIF-B cells, the hepatoma-derived hybrid cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Oct 17 2016

Question
Answer

DISCOUNT CODES:

For Ab17377: ***
For Ab96196: ***

Expiration date: February 18, 2013

Value: $337 each

Each of these codes will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for each of these antibodies and include the corresponding code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Each code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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