Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)

Reviews (5)Q&A (1)References (6)
Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)
  • Immunocytochemistry/ Immunofluorescence - Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)

Key features and details

  • Rabbit polyclonal to Polymeric immunoglobulin receptor/PIGR
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Polymeric immunoglobulin receptor/PIGR antibody
    See all Polymeric immunoglobulin receptor/PIGR primary antibodies

  • Description

    Rabbit polyclonal to Polymeric immunoglobulin receptor/PIGR

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human Polymeric immunoglobulin receptor/PIGR aa 180-429.

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Molt4 and Raji cells; Cal27 xenograft tissue.

  • General notes

    Previously labelled as PIGR. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/500 - 1/300. Predicted molecular weight: 83 kDa.
IHC-P
1/100 - 1/500.
ICC/IF (2)
1/100 - 1/200.
Notes
WB
1/500 - 1/300. Predicted molecular weight: 83 kDa.
IHC-P
1/100 - 1/500.
ICC/IF
1/100 - 1/200.

Target

  • Function

    This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

  • Sequence similarities

    Contains 5 Ig-like V-type (immunoglobulin-like) domains.

  • Post-translational
    modifications

    N-glycosylation is not necessary for Ig binding.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Hepatocellular carcinoma associated protein TB6 antibody
      • Hepatocellular carcinoma-associated protein TB6 antibody
      • MGC125361 antibody
      • MGC125362 antibody
      • Phosphatidylinositol glycan, class R antibody
      • PIGR antibody
      • PIGR_HUMAN antibody
      • Poly Ig receptor antibody
      • Poly-Ig receptor antibody
      • Polymeric immunoglobulin receptor antibody
      • Secretory component antibody
      • Transmembrane secretory component antibody
      see all

    Images

    • Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)
      Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)
      Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg

      Predicted band size: 83 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)
      Immunocytochemistry/ Immunofluorescence - Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)

      ab96196 at 1/200 dilution staining Polymeric immunoglobulin receptor/PIGR in methanol fixed HepG2 cells. Lower image is co-stained with Hoechst 33342.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)

      ab96196 at 1/500 dilution staining Polymeric immunoglobulin receptor/PIGR in paraffin-embedded Cal27 xenograft tissue.

    References (6)

    Publishing research using ab96196? Please let us know so that we can cite the reference in this datasheet.

    ab96196 has been referenced in 6 publications.

    • Ohkuma R  et al. High expression levels of polymeric immunoglobulin receptor are correlated with chemoresistance and poor prognosis in pancreatic cancer. Oncol Rep 44:252-262 (2020). PubMed: 32627041
    • Deleon-Pennell KY  et al. Glycoproteomic Profiling Provides Candidate Myocardial Infarction Predictors of Later Progression to Heart Failure. ACS Omega 4:1272-1280 (2019). PubMed: 30729226
    • Zhou M  et al. Expression of polymeric immunoglobulin receptor and its biological function in endometrial adenocarcinoma. J Cancer Res Ther 15:420-425 (2019). PubMed: 30964121
    • Huang L  et al. A study on the mechanism of agonists in regulating transcriptional level of pIgR in salivary gland epithelial cells. Exp Ther Med 16:4367-4372 (2018). PubMed: 30542385
    • Minghui Z  et al. Analysis of Differentially Expressed Proteins Involved in Autoimmune Cirrhosis and Normal Serum by iTRAQ Proteomics. Proteomics Clin Appl N/A:e1700153 (2018). PubMed: 29999587
    • Casey CA  et al. Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells. Biomolecules 8:N/A (2018). PubMed: 30453527

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Polymeric immunoglobulin receptor/PIGR antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat Hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Rat Hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Dec 21 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Polymeric immunoglobulin receptor/PIGR antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (WIF-B cells, the hepatoma-derived hybrid cell line)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    WIF-B cells, the hepatoma-derived hybrid cell line
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Oct 17 2016

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