Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)
Key features and details
- Rabbit polyclonal to Polymeric immunoglobulin receptor/PIGR
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Polymeric immunoglobulin receptor/PIGR antibody
See all Polymeric immunoglobulin receptor/PIGR primary antibodies -
Description
Rabbit polyclonal to Polymeric immunoglobulin receptor/PIGR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Polymeric immunoglobulin receptor/PIGR aa 180-429.
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt4 and Raji cells; Cal27 xenograft tissue.
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General notes
Previously labelled as PIGR.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
1/500 - 1/300. Predicted molecular weight: 83 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF | (2) |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/300. Predicted molecular weight: 83 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
Target
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Function
This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment. -
Sequence similarities
Contains 5 Ig-like V-type (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylation is not necessary for Ig binding. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5284 Human
- Omim: 173880 Human
- SwissProt: P01833 Human
- Unigene: 497589 Human
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Alternative names
- Hepatocellular carcinoma associated protein TB6 antibody
- Hepatocellular carcinoma-associated protein TB6 antibody
- MGC125361 antibody
see all
Images
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Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg
Predicted band size: 83 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)
ab96196 at 1/200 dilution staining Polymeric immunoglobulin receptor/PIGR in methanol fixed HepG2 cells. Lower image is co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody (ab96196)
ab96196 at 1/500 dilution staining Polymeric immunoglobulin receptor/PIGR in paraffin-embedded Cal27 xenograft tissue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab96196 has been referenced in 6 publications.
- Ohkuma R et al. High expression levels of polymeric immunoglobulin receptor are correlated with chemoresistance and poor prognosis in pancreatic cancer. Oncol Rep 44:252-262 (2020). PubMed: 32627041
- Deleon-Pennell KY et al. Glycoproteomic Profiling Provides Candidate Myocardial Infarction Predictors of Later Progression to Heart Failure. ACS Omega 4:1272-1280 (2019). PubMed: 30729226
- Zhou M et al. Expression of polymeric immunoglobulin receptor and its biological function in endometrial adenocarcinoma. J Cancer Res Ther 15:420-425 (2019). PubMed: 30964121
- Huang L et al. A study on the mechanism of agonists in regulating transcriptional level of pIgR in salivary gland epithelial cells. Exp Ther Med 16:4367-4372 (2018). PubMed: 30542385
- Minghui Z et al. Analysis of Differentially Expressed Proteins Involved in Autoimmune Cirrhosis and Normal Serum by iTRAQ Proteomics. Proteomics Clin Appl N/A:e1700153 (2018). PubMed: 29999587
- Casey CA et al. Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells. Biomolecules 8:N/A (2018). PubMed: 30453527