Key features and details
- Suitable for: ELISA
Product namePolymyxin B peptide
Purity70 - 90% by HPLC.
Our Abpromise guarantee covers the use of ab85004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesab85007 was expressed by fermentation of Bacillus polymyxa. The peptide was subsequently purified by HPLC.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
ReconstitutionReconstitute the vial with distilled or deionized water, volume depending on the concentration to be used. After reconstitution store stock solution in aliquots at -20°C.
RelevanceThe peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab85004 has not yet been referenced specifically in any publications.