Overview

  • Product name
    Anti-Polyoma virus, Large T antigen antibody [PyLT]
  • Description
    Rat monoclonal [PyLT] to Polyoma virus, Large T antigen
  • Host species
    Rat
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Immunogen

    Polyoma virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A/G purified
  • Clonality
    Monoclonal
  • Clone number
    PyLT
  • Myeloma
    NS1
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 100 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    The mouse polyoma virus (Py) is an oncogenic virus, which encodes for three early proteins, large, middle and small T (tumour) antigen. PyLT allows isolation of viral T antigens.

References

This product has been referenced in:
  • Dilworth SM Protein kinase activities associated with distinct antigenic forms of polyoma virus middle T-antigen. EMBO J 1:1319-28 (1982). Read more (PubMed: 6327264) »
  • Dilworth SM & Griffin BE Monoclonal antibodies against polyoma virus tumor antigens. Proc Natl Acad Sci U S A 79:1059-63 (1982). Read more (PubMed: 6175960) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your telephone enquiry earlier this afternoon. I am sorry to confirm we have no data on whether this antibody would crossreact wtih SV40 and regrettably the epitope has not been mapped. We do have the following 2 publications for this antibody, which may be helpful: 0030.pdf The EMBO Journal Vol.1 No.1I1 pp. 1319-1328, 1982 Protein kinase activities associated with distinct antigenic forms of polyoma virus middle T-antigen Stephen M.Dilworth* http://ops.adminsite.com/admin/abcamshare/CRM/attach/2011/7/22/CCE3003928_pnas00443-0126.pdf Proc. Natl Acad. Sci. USA Vol. 79, pp. 1059-1063, February 1982 Biochemistry Monoclonal antibodies against polyoma virus tumor antigens (middle tumor antigen/large tumor antigen/rat IgG2b/antigen heterogeneity) STEPHEN M. DILWORTH AND BEVERLY E. GRIFFIN I am sorry we are not able to provide the information you require on this occasion. If you have any further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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