Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085)

Abreviews (1)Q&A (7)Specific References (9)

Overview

  • Product name
    Anti-Polyoma virus, Medium T antigen antibody [PyMT]
  • Description
    Rat monoclonal [PyMT] to Polyoma virus, Medium T antigen
  • Host species
    Rat
  • Tested applications
    Suitable for: IP, ICC/IF, WBmore details
  • Immunogen

    Synthetic peptide (N terminal).

  • General notes


    Binds medium T antigen only, allows isolation of viral T antigens.

Properties

Applications

Our Abpromise guarantee covers the use of ab15085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent concentration.

Target

  • Relevance
    Middle T antigen (MT) is a 421-amino-acid protein associated with membranes and underlying cytoskeletal elements, and is associated with a tyrosine-specific protein kinase activity. It is the principal oncoprotein of polyomavirus that is necessary and often sufficient for transformation in vitro. MT delivered as a transgene or a retrovirus can induce tumors in a wide variety of tissues. Polyomavirus (PyV) is a small, double-stranded, closed-circular-DNA virus with an approximately 5-kb genome divided into two roughly equal regions. The late transcripts produce the viral capsid proteins, whereas the early region encodes three so-called tumor (T) antigens that are important for both productive infection and transformation.
  • Cellular localization
    Cytoplasmic location in cells infected with virus.
  • Alternative names
    • Middle T antigen antibody
    • MT AG antibody
    • MT antibody
    • Polyoma virus middle t antigen antibody

References

This product has been referenced in:
  • Kim J  et al. Ablation of miR-10b Suppresses Oncogene-Induced Mammary Tumorigenesis and Metastasis and Reactivates Tumor-Suppressive Pathways. Cancer Res 76:6424-6435 (2016). ICC/IF . Read more (PubMed: 27569213) »
  • Roy A  et al. Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma. PLoS One 11:e0156151 (2016). Read more (PubMed: 27223472) »

See all 9 Publications for this product

Publishing research using ab15085? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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8 reviews or Q&A

10
Votes: Highest First

Product code: 15085
Lot number: GR37142-3

Inquiry: I would like to have some suggestion regarding the use of the above mentioned Ab in immunofluorescence. We stain the lungs to look for metastasis and visualize it with Alexa568 secondary. ...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am at...

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Phone call requesting information of how the Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085) has been used in ICC.

I am sorry for the delay in getting back to you in regards to the protocol used with the Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085).
I can now provide some further guidance in how I would suggest using the antibody in IHC. From...

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Phone call requesting information of how the Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085) has been used in ICC.

Thank you for contacting us yesterday in regards to the Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085).
Unfortunately, as we discussed over the phone, this antibody has as yet not been used in IHC to our knowledge. This does not me...

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Please see technical inquiry from our customer:

I’m interested in product ab15085 Anti-Polyoma virus, Medium T antigen antibody [PyMT].

I like to know whether this antibody will also be able to detect the Large and small T antig...

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Thank you very much for your patience.
We can confirm that the antibody is specific to Medium T form only. We have no cross reactivity data regarding small and large T forms. The clone specific publication data show the antibody should not be cross...

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Hi,
we are using antibody ab15085 for IF on formalin-fixed paraffin-embedded tissues, but we always get tons of autofluorescence. do you have good protocol for this?
btw, does this antibody can be used for IHC? or any other polyomavirious mid...

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Thank you for contacting us.

I am afraid ab15085 has not been tested in IHC, and therefore we cannot guarantee it will work in this application. You may find useful our online protocols about IHC in order to optimise the staining:

h...

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Western blot abreview for Anti-Polyoma virus, Medium T antigen antibody [PyMT]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Breast tumor)
Loading amount
50 µg
Specification
Breast tumor
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Verified customer

Submitted Jan 27 2011

Lot # 145691 I think that the purchase order number is UR114911, if not then ill talk to the administrators The PO number is 346756

Thank you for your email and patience. I presently have concerns about the suitability of this antibody in Western blotting and have removed the application from our online datasheet. We haven't received any other complaints, but the originator of this...

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Are there more details available about how this antibody was tested in histology?

Thank you for your enquiry. More details about this antibody can be found in the following references: Dilworth SM & Griffin BE Monoclonal antibodies against polyoma virus tumor antigens. Proc Natl Acad Sci U S A 79:1059-63 (1982). PubMed: 6175960 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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