Overview

  • Product name

    Anti-Polyoma virus, Medium T antigen antibody [PyMT]
  • Description

    Rat monoclonal [PyMT] to Polyoma virus, Medium T antigen
  • Host species

    Rat
  • Tested applications

    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Synthetic peptide (N terminal).

  • General notes


    Binds medium T antigen only, allows isolation of viral T antigens.

Properties

Applications

Our Abpromise guarantee covers the use of ab15085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent concentration.

Target

  • Relevance

    Middle T antigen (MT) is a 421-amino-acid protein associated with membranes and underlying cytoskeletal elements, and is associated with a tyrosine-specific protein kinase activity. It is the principal oncoprotein of polyomavirus that is necessary and often sufficient for transformation in vitro. MT delivered as a transgene or a retrovirus can induce tumors in a wide variety of tissues. Polyomavirus (PyV) is a small, double-stranded, closed-circular-DNA virus with an approximately 5-kb genome divided into two roughly equal regions. The late transcripts produce the viral capsid proteins, whereas the early region encodes three so-called tumor (T) antigens that are important for both productive infection and transformation.
  • Cellular localization

    Cytoplasmic location in cells infected with virus.
  • Database links

    • Alternative names

      • Middle T antigen antibody
      • MT AG antibody
      • MT antibody
      • Polyoma virus middle t antigen antibody

    References

    This product has been referenced in:

    See all 11 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results.

    This information will also allow us to investigate this case internally and initiate additional testing where necessary.

    I look forward to receiving your reply.

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    Answer

    I am sorry for the delay in getting back to you in regards to the protocol used with the Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085).
    I can now provide some further guidance in how I would suggest using the antibody in IHC. From the information I have I would suggest initially trying the antibody at a dilution of 1/100. From this you should be able to ascertain if you can reduce the concentration further, or if you do not get any signal, you may have to decrease the dilution.
    As we discussed over the phone, antigen retrieval may be required and this may need some optimisation to get the best results. We have quite a comprehensive guide which can give you a few tips on how this can be approached:
    https://docs.abcam.com/pdf/misc/abcam-protocols-book-2010.pdf
    If I can be of any further assistance, please do let me know. Until then, I wish you all the best with your research.

    Read More

    Answer

    Thank you for contacting us yesterday in regards to the Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085).
    Unfortunately, as we discussed over the phone, this antibody has as yet not been used in IHC to our knowledge. This does not mean that it would not work, simply that we have not tried it ourselves and would not be able to guarantee its performance in this application. However, if you would like to purchase ab15085 and use it in IHC-P, we can offer you a testing discount. This offer means that if you purchase ab15085 as normal, test the antibody in IHC-P and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab15085 off any product). More information on this offer can be found here:
    www.abcam.com/collaborationdiscount
    Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.
    I have generated the testing discount code as follows:
    Discount code: xxxxx
    Expiration date: 16th of January 2013.
    Please quote this code in your Abreview for ab15085 to activate it.
    As discussed over the phone, I am seeking further information as to how the antibody was used in ICC and will forward this information to you once I have it.
    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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    Answer

    Thank you very much for your patience.
    We can confirm that the antibody is specific to Medium T form only. We have no cross reactivity data regarding small and large T forms. The clone specific publication data show the antibody should not be cross reacting with the small and long forms.
    I hope this information is nevertheless helpful to you . Should you have any question please do not hesitate to contact me.

    Read More

    Answer

    Thank you for contacting us.

    I am afraid ab15085 has not been tested in IHC, and therefore we cannot guarantee it will work in this application. You may find useful our online protocols about IHC in order to optimise the staining:

    https://www.abcam.com/index.html?pageconfig=popular_protocols

    Unfortunately this is the only antibody available against the Medium T antigen from Polyoma virus.

    Autofluorescence may be a consequence of the tissue itself, or even the fixative. You can try to optimise the results by reducing antibody concentration, washing the slides adequately, use serum from the species in which the secondary was raised to block unspecific bindings and depending on the tissue and the conditions used, blocking of endogenous enzymes may also be useful.

    It is also crucial to use a positive control to assess the source of the problem

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Breast tumor)
    Loading amount
    50 µg
    Specification
    Breast tumor
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 27 2011

    Answer

    Thank you for your email and patience. I presently have concerns about the suitability of this antibody in Western blotting and have removed the application from our online datasheet. We haven't received any other complaints, but the originator of this antibody has told me that it works "weakly" in WB and there is no additional information regarding this. I would be happy to offer you a credit note or refund, please let me know which you would prefer and I look forward to hearing from you. Have a great weekend.

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    Question
    Answer

    Thank you for your enquiry. More details about this antibody can be found in the following references: Dilworth SM & Griffin BE Monoclonal antibodies against polyoma virus tumor antigens. Proc Natl Acad Sci U S A 79:1059-63 (1982). PubMed: 6175960 Dilworth SM Protein kinase activities associated with distinct antigenic forms of polyoma virus middle T-antigen. EMBO J 1:1319-28 (1982). PubMed: 6327264

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