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we are using antibody ab15085 for IF on formalin-fixed paraffin-embedded tissues, but we always get tons of autofluorescence. do you have good protocol for this?
btw, does this antibody can be used for IHC? or any other polyomavirious middle T antigen antibody can work for IHC?
thanks a lot.
Asked on Apr 02 2012
Thank you for contacting us.
I am afraid ab15085 has not been tested in IHC, and therefore we cannot guarantee it will work in this application. You may find useful our online protocols about IHC in order to optimise the staining:
Unfortunately this is the only antibody available against the Medium T antigen from Polyoma virus.
Autofluorescence may be a consequence of the tissue itself, or even the fixative. You can try to optimise the results by reducing antibody concentration, washing the slides adequately, use serum from the species in which the secondary was raised to block unspecific bindings and depending on the tissue and the conditions used, blocking of endogenous enzymes may also be useful.
It is also crucial to use a positive control to assess the source of the problem
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Apr 02 2012