Product namePolysucrose 400, synthetic sucrose polymer
DescriptionNonionic synthetic polymer of sucrose.
- Ficoll 400
Synthetic high molecular weight polymer made by the copolymerization of sucrose and epichlorohydrin. The molecules have a branched structure with a high content of hydroxyl groups giving a good solubility in aqueous solutions. Used for the density gradient separation of cells, cell membranes and virus cells. It can also be used to isolate different organelles. Has been used in a study to develop a new method for the purification of human motile spermatozoa.
Molecular weight: 350000-500000
Storage instructionsStore at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in water to 100 mg/ml
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Graham JM Isolation of peripheral blood mononuclear cells from macaques on a density barrier. ScientificWorldJournal 2:1654-6 (2002). Read more (PubMed: 12806157) »
- Andersen CY & Grinsted J A new method for the purification of human motile spermatozoa applying density-gradient centrifugation: polysucrose media compared to Percoll media. J Assist Reprod Genet 14:624-8 (1997). Read more (PubMed: 9447466) »
- Oman H et al. Chemical and physiological properties of polysucrose, a new marker of intestinal permeability to macromolecules. Int Arch Allergy Immunol 98:220-6 (1992). Read more (PubMed: 1392623) »