Key features and details
- Rabbit polyclonal to Polyubiquitin-C
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Polyubiquitin-C antibody
See all Polyubiquitin-C primary antibodies
DescriptionRabbit polyclonal to Polyubiquitin-C
SpecificityThis Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids of human Ubiquitin.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Polyubiquitin-C aa 36-66 (C terminal).
- Human Prostate and Testis tissue
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab104455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 15 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceUbiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Cellular localizationCytoplasm. Nucleus
- HMG20 antibody
- Polyubiquitin-C antibody
- UBC antibody
- Ubiquitin antibody
ab104455, at 10 µg/ml, staining Polyubiquitin-C in formalin-fixed, paraffin-embedded Human Prostate by Immunohistochemistry, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab104455, at 10 µg/ml, staining Polyubiquitin-C in formalin-fixed, paraffin-embedded Human Testis by Immunohistochemistry, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis of anti-Ubiquitin Pab (ab104455) in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
ab104455 has not yet been referenced specifically in any publications.