Overview

  • Product name
    Anti-POMC antibody
    See all POMC primary antibodies
  • Description
    Goat polyclonal to POMC
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    C-NAIIKNAYKKGE

    , corresponding to C terminal amino acids 256-267 of Human POMC.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human lymph node and murine pituitary cell line NIH 3T3 lysates. Human adrenal gland tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab32893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).

This product appears to react with the full length precursor protein only.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/75. PubMed: 23469282
IHC-FoFr Use at an assay dependent concentration. PubMed: 21526203

Target

  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ACTH antibody
    • Adrenocorticotropic hormone antibody
    • Adrenocorticotropin antibody
    • alpha melanocyte stimulating hormone antibody
    • Alpha MSH antibody
    • Alpha-MSH antibody
    • beta endorphin antibody
    • Beta LPH antibody
    • beta melanocyte stimulating hormone antibody
    • Beta MSH antibody
    • Beta-LPH antibody
    • Beta-MSH antibody
    • CLIP antibody
    • COLI_HUMAN antibody
    • Corticotropin like intermediary peptide antibody
    • Corticotropin lipotropin antibody
    • Corticotropin-lipotropin antibody
    • Gamma MSH antibody
    • Gamma-LPH antibody
    • Gamma-MSH antibody
    • Lipotropin beta antibody
    • Lipotropin gamma antibody
    • LPH antibody
    • Melanotropin alpha antibody
    • Melanotropin antibody
    • Melanotropin beta antibody
    • Melanotropin gamma antibody
    • met enkephalin antibody
    • Met-enkephalin antibody
    • MSH antibody
    • NPP antibody
    • POC antibody
    • POMC antibody
    • pro ACTH endorphin antibody
    • Proopiomelanocortin antibody
    • Proopiomelanocortin preproprotein antibody
    see all

Images

  • Western blot - Anti-POMC antibody (ab32893)
    Western blot - Anti-POMC antibody (ab32893)
    Anti-POMC antibody (ab32893) at 0.01 µg/ml + NIH3T3 cell lysate (35µg in RIPA buffer)

    Predicted band size: 29 kDa
    Observed band size: 26 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.
    Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)
    Immunohistochemical analysis of Human pituitary gland labeling POMC with ab32893. Antigen retrieval by steaming with Tris/EDTA buffer pH 9. HRP-staining. 

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)

    ab32893 (4µg/ml) staining of POMC in paraffin embedded Human Adrenal Gland following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining shows vesiculate staining in some glandular cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)

    ab32893 at 2.5ug/ml staining of paraffin embedded human adrenal gland tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)
    ab32893 (4µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Shows vesiculate staining in some glandular cells.

References

This product has been referenced in:

See all 11 Publications for this product

Publishing research using ab32893? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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9 reviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-POMC antibody

 Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Adrenal gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Adrenal gland
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% Normal buffered formalin
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Abcam user community

Verified customer

Submitted Dec 06 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-POMC antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin
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Username

Abcam user community

Verified customer

Submitted Dec 06 2016


My lab recently purchased an antibody from abcam (Anti-POMC, ab 32893) which in the species reactivity section said "predicted to react with rat due to sequence homology". I needed it to do an IHC on frozen rat brain sections, I tried al...

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Thank you for contacting Abcam and for providing that valuable feedback on ab32893.

You could submit an Abreview on this product (following the link below), that way you could earn some reward points:

https://www.abcam.com/index.htm...

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I received the new vial of POMC antibody and tried it at the weekend. It turns out that this new vial still does not work well.   So we decided to ask for the refund for the original puchase.

Thank you for contacting us. Your credit note ID is XXX. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of...

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I would like to take the a free of charge replacement first, ifthe new vial still doesnot work, then I will ask for refund. Is this ok?

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience th...

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Protein Blocking step:  5%NGS in PBS, RT 1h.   peroxidase quencing:  3% H2O2 in PBS     Yes, I saw the paper, that's why I ordered this antibody. But unfortunately, it really doesn't work on our hand. And we have already checked that paper for mor...

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Thank you for taking time to send me the details of your protocol and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide ...

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Please see attached for details about ab32893

Thank you for providing this further information.

I appreciate your cooperation and understand your concerns. It is regrettable the results have not been succesful.

In order to help with our investigation of this case before d...

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Hello, The antibody (ab32893) we order doesnot work, without any signaling. The P.O. number is What should we do?

Thank you for contacting Abcam.

I am sorry to hear of the difficulties that you have been experiencing using these products. Would you mind sending me the details of your protocol including the tissue type you are working with, your sample p...

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We would like to do stainings of POMC neurons in the hypothalamus of paraffin embedded mouse brain sections. As an example you show a staining of human adrenal gland tissue with the above mentioned antibody on your website. We are now wondering if the ...

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Thank you for your enquiry. All of the information we have for each of our products is listed on the online datasheet for your convenience and these are updated as soon as we receive additional data. Unfortunately, we currently do not have any info...

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