Anti-POMC antibody (ab32893)

Reviews (5)Q&A (7)References (19)

Key features and details

  • Goat polyclonal to POMC
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-POMC antibody
    See all POMC primary antibodies

  • Description

    Goat polyclonal to POMC

  • Host species

    Goat

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide corresponding to Human POMC aa 256-267 (C terminal) (Cysteine residue).
    Sequence:

    C-NAIIKNAYKKGE


    Database link: P01189

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab32893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).

A 1 hour primary incubation is recommended for this product. This product appears to react with the full length precursor protein only.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ACTH antibody
    • Adrenocorticotropic hormone antibody
    • Adrenocorticotropin antibody
    • alpha melanocyte stimulating hormone antibody
    • Alpha MSH antibody
    • Alpha-MSH antibody
    • beta endorphin antibody
    • Beta LPH antibody
    • beta melanocyte stimulating hormone antibody
    • Beta MSH antibody
    • Beta-LPH antibody
    • Beta-MSH antibody
    • CLIP antibody
    • COLI_HUMAN antibody
    • Corticotropin like intermediary peptide antibody
    • Corticotropin lipotropin antibody
    • Corticotropin-lipotropin antibody
    • Gamma MSH antibody
    • Gamma-LPH antibody
    • Gamma-MSH antibody
    • Lipotropin beta antibody
    • Lipotropin gamma antibody
    • LPH antibody
    • Melanotropin alpha antibody
    • Melanotropin antibody
    • Melanotropin beta antibody
    • Melanotropin gamma antibody
    • met enkephalin antibody
    • Met-enkephalin antibody
    • MSH antibody
    • NPP antibody
    • POC antibody
    • POMC antibody
    • pro ACTH endorphin antibody
    • Proopiomelanocortin antibody
    • Proopiomelanocortin preproprotein antibody
    see all

Images

  • Western blot - Anti-POMC antibody (ab32893)
    Western blot - Anti-POMC antibody (ab32893)
    Lane 1 : Anti-POMC antibody (ab32893) at 0.3 µg/ml
    Lane 2 : Anti-POMC antibody (ab32893) at 2 µg/ml

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)
    Immunohistochemical analysis of Human pituitary gland labeling POMC with ab32893. Antigen retrieval by steaming with Tris/EDTA buffer pH 9. HRP-staining. 

     

References (19)

Publishing research using ab32893? Please let us know so that we can cite the reference in this datasheet.

ab32893 has been referenced in 19 publications.

  • Kameda H  et al. Proton Sensitivity of Corticotropin-Releasing Hormone Receptor 1 Signaling to Proopiomelanocortin in Male Mice. Endocrinology 160:276-291 (2019). PubMed: 30535142
  • Fukuhara S  et al. High-fat diet accelerates extreme obesity with hyperphagia in female heterozygous Mecp2-null mice. PLoS One 14:e0210184 (2019). PubMed: 30608967
  • He Y  et al. Child compound Endothelium corneum attenuates gastrointestinal dysmotility through regulating the homeostasis of brain-gut-microbiota axis in functional dyspepsia rats. J Ethnopharmacol 240:111953 (2019). PubMed: 31082513
  • Zhou YZ  et al. The Effects of Smad3 on Adrenocorticotropic Hormone-Secreting Pituitary Adenoma Development, Cell Proliferation, Apoptosis, and Hormone Secretion. World Neurosurg 114:e329-e337 (2018). WB . PubMed: 29524699
  • Gaspar JM  et al. Downregulation of HIF complex in the hypothalamus exacerbates diet-induced obesity. Brain Behav Immun 73:550-561 (2018). PubMed: 29935943
View all Publications for this product

Customer reviews and Q&As

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1-10 of 12 Abreviews or Q&A

Immunohistochemistry free floating abreview for Anti-POMC antibody

 Poor
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (brain)
Specification
brain
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Abcam user community

Verified customer

Submitted Sep 09 2019

Immunohistochemistry free floating abreview for Anti-POMC antibody

 Poor
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain
Read More

Abcam user community

Verified customer

Submitted Jan 23 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-POMC antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat brain tissue)
Permeabilization
Yes - 0.3% Triton X-100
Specification
Rat brain tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 10 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-POMC antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Adrenal gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Adrenal gland
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% Normal buffered formalin
Read More

Abcam user community

Verified customer

Submitted Dec 06 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-POMC antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin
Read More

Abcam user community

Verified customer

Submitted Dec 06 2016

Question


My lab recently purchased an antibody from abcam (Anti-POMC, ab 32893) which in the species reactivity section said "predicted to react with rat due to sequence homology". I needed it to do an IHC on frozen rat brain sections, I tried all different dilutions from 1:200 to 1:400 using standard IHC protocol and I didn't see staining in any of my sections (esp. in general hypothalamus area). Therefore I believe this antibody doesn't react with rat and it'd be nice if you will remove that prediction from this antibody. Also I didn't see any special pre-treatment on the data sheet for this antibody, if there is one, I'd appreciate it if you'll let me know and I'll try it out.

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Answer

Thank you for contacting Abcam and for providing that valuable feedback on ab32893.

You could submit an Abreview on this product (following the link below), that way you could earn some reward points:

https://www.abcam.com/index.html?pageconfig=resource&rid=10332

Also, in future, we do have a special 100% Abreview Testing Discount program for instances like this, where we cannot guarantee that an antibody would work in a particular application or species:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

All of the information we have concerning ab32893 is on the webpages and I cannot find any particular pre-treatment for frozen tissue that is recommended to use with this antibody.

Please let me know if there is anything else I can help you with.

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Question

I received the new vial of POMC antibody and tried it at the weekend. It turns out that this new vial still does not work well.   So we decided to ask for the refund for the original puchase.  

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Answer

Thank you for contacting us. Your credit note ID is XXX. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Question

I would like to take the a free of charge replacement first, ifthe new vial still doesnot work, then I will ask for refund. Is this ok?

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1070241.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. We will be happy to refund your original purchase.

I wish you the best of luck with your research.

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Question

Protein Blocking step:  5%NGS in PBS, RT 1h.   peroxidase quencing:  3% H2O2 in PBS     Yes, I saw the paper, that's why I ordered this antibody. But unfortunately, it really doesn't work on our hand. And we have already checked that paper for more details of protocol, but still didnot work.

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Answer

Thank you for taking time to send me the details of your protocol and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details and images, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Question

Please see attached for details about ab32893

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Answer

Thank you for providing this further information.

I appreciate your cooperation and understand your concerns. It is regrettable the results have not been succesful.

In order to help with our investigation of this case before deciding how to proceed, I would appreciate if you are able to provide more detail as requested in the previous email. This information will also be helpful to our quality monitoring.

Could you please tell me about your blocking steps? Do you endogenous peroxidases? What are the protein blocking buffer used and the incubation times?

This product has been tested and published with in IHC-FoFr on mouse (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=21526203&dopt=Abstract). I will be pleased to provide a free of charge replacmenet or credit note if the antibody is not working with the suggestions provided.


Thank you for your time. I look forward to hearing from you with the requested information and hope we can resolve this case as soon as possible.

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1-10 of 12 Abreviews or Q&A

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