Anti-POMC antibody (ab32893)
Key features and details
- Goat polyclonal to POMC
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-POMC antibody
See all POMC primary antibodies -
Description
Goat polyclonal to POMC -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human POMC aa 256-267 (C terminal) (Cysteine residue).
Sequence:C-NAIIKNAYKKGE
Database link: P01189 -
Positive control
- WB: NIH/3T3 cell lysate and rat brain tissue lysate; IHC-P: Human pituitary gland; Flow Cyt (intra): A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
A 1 hour primary incubation is recommended for this product. This product appears to react with the full length precursor protein only. |
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| IHC-P | (2) |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa). A 1 hour primary incubation is recommended for this product. This product appears to react with the full length precursor protein only. |
|
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 5443 Human
- Entrez Gene: 18976 Mouse
- Entrez Gene: 24664 Rat
- Omim: 176830 Human
- SwissProt: P01189 Human
- SwissProt: P01193 Mouse
- SwissProt: P01194 Rat
- Unigene: 1897 Human
see all -
Alternative names
- ACTH antibody
- Adrenocorticotropic hormone antibody
- Adrenocorticotropin antibody
see all
Images
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Western blot - Anti-POMC antibody (ab32893)Lane 1 : Anti-POMC antibody (ab32893) at 0.3 µg/ml
Lane 2 : Anti-POMC antibody (ab32893) at 2 µg/ml
Lane 1 : NIH3T3 cell lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 29 kDaPrimary incubation 1 hour at room temperature.
Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (ab32893)Immunohistochemical analysis of Human pituitary gland labeling POMC with ab32893. Antigen retrieval by steaming with Tris/EDTA buffer pH 9. HRP-staining. -
Flow Cytometry (Intracellular) - Anti-POMC antibody (ab32893)Flow cytometry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells (blue line) labeling POMC with ab32893 at 10 µg/mL. Cells permeabilized with 0.5% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 1 µg/mL.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (25)
ab32893 has been referenced in 25 publications.
- Xia L et al. Targeting the TR4 nuclear receptor with antagonist bexarotene can suppress the proopiomelanocortin signalling in AtT-20 cells. J Cell Mol Med 25:2404-2417 (2021). PubMed: 33491272
- Lee EY et al. The local hypothalamic-pituitary-adrenal axis in cultured human dermal papilla cells. BMC Mol Cell Biol 21:42 (2020). PubMed: 32522165
- Wu Z et al. Deletion of liver kinase B1 in POMC neurons predisposes to diet-induced obesity. Life Sci 258:118204 (2020). PubMed: 32763296
- Alkan I et al. Is vagal stimulation or inhibition benefit on the regulation of the stomach brain axis in obesity? Nutr Neurosci N/A:1-13 (2020). PubMed: 33034260
- Zhang S et al. Single-cell transcriptomics identifies divergent developmental lineage trajectories during human pituitary development. Nat Commun 11:5275 (2020). PubMed: 33077725
