Anti-POMK antibody (ab272564)
Key features and details
- Rabbit polyclonal to POMK
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-POMK antibody -
Description
Rabbit polyclonal to POMK -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human POMK aa 200-350.
Database link: Q9H5K3 -
Positive control
- WB: HEK-293T cells. IHC-P: Human placenta tissue. ICC/IF: U-251 MG.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif. -
Involvement in disease
Muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies A12 -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. STKL subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 84197 Human
- Omim: 615247 Human
- SwissProt: Q9H5K3 Human
- Unigene: 491646 Human
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Alternative names
- FLJ23356 antibody
- MDDGA12 antibody
- POMK antibody
see all
Images
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Lanes 2-3 : Anti-POMK antibody (ab272564) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : Control (vector only transfected HEK-293T lysate)
Lane 3 : POMK over-expression lysate (mammalian HEK-293T cells) -
Paraffin-embedded formalin-fixed Human placenta tissue stained for POMK using ab272564 at 1/50 in immunohistochemical analysis.
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PFA fixed, Triton X-100 permeablized human cell line U-251 MG labeling POMK (green) using ab272564 at 2 ug/ml in ICC/IF analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272564 has not yet been referenced specifically in any publications.