Anti-PON1 antibody (ab228597)

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Overview

  • Product name

    Anti-PON1 antibody
    See all PON1 primary antibodies

  • Description

    Rabbit polyclonal to PON1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant full length protein corresponding to Human PON1 aa 1-355.
    Sequence:

    MAKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIET GSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLEL GITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEE KSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLQSWEMYLGL AWSYVVYYSPSEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEK HANWTLTPLKSLDFNTLVDNISVDPETGDLWVGCHPNGMKIFFYDSENPP ASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKA LYCEL


    Database link: P27169

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Human liver tissue extracts and plasma. IHC-P: Mouse liver tissue.

Applications

Our Abpromise guarantee covers the use of ab228597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.

Target

  • Function

    Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.

  • Tissue specificity

    Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.

  • Involvement in disease

    Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.

  • Sequence similarities

    Belongs to the paraoxonase family.

  • Post-translational
    modifications

    Glycosylated.
    The signal sequence is not cleaved.
    Present in two forms, form B contains a disulfide bond, form A does not.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • A esterase 1 antibody
      • A-esterase 1 antibody
      • Aromatic esterase 1 antibody
      • Arylesterase 1 antibody
      • Arylesterase B type antibody
      • ESA antibody
      • Esterase A antibody
      • K 45 antibody
      • K-45 antibody
      • MVCD5 antibody
      • Paraoxonase 1 antibody
      • Paraoxonase antibody
      • Paraoxonase B type antibody
      • Paraoxonase, plasma antibody
      • Paraoxonase1 antibody
      • PON 1 antibody
      • PON antibody
      • PON1 antibody
      • PON1_HUMAN antibody
      • Serum aryldiakylphosphatase antibody
      • Serum aryldialkylphosphatase 1 antibody
      • Serum paraoxonase/arylesterase 1 antibody
      see all

    Images

    • Western blot - Anti-PON1 antibody (ab228597)
      Western blot - Anti-PON1 antibody (ab228597)
      All lanes : Anti-PON1 antibody (ab228597) at 1/500 dilution

      Lane 1 : Human plasma
      Lane 2 : Human liver tissue extract

      Lysates/proteins at 30 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 40 kDa



      10% SDS-PAGE

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody (ab228597)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody (ab228597)

      Paraffin-embedded mouse liver tissue stained for PON1 with ab228597 at 1/500 dilution in immunohistochemical analysis.

    References

    ab228597 has not yet been referenced specifically in any publications.

    Publishing research using ab228597? Please let us know so that we can cite the reference in this datasheet.

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