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    pon1-antibody-ab99037.pdf

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Anti-PON1 antibody (ab99037)

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Western blot - Anti-PON1 antibody (ab99037)

    Key features and details

    • Rabbit polyclonal to PON1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant Human PON1 protein (ab53376)

    View more associated products

    Overview

    • Product name

      Anti-PON1 antibody
      See all PON1 primary antibodies
    • Description

      Rabbit polyclonal to PON1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Chimpanzee, Macaque monkey, Gorilla
    • Immunogen

      Synthetic peptide corresponding to Human PON1 aa 150-250 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab123820)

    • Positive control

      • This antibody gave a positive signal in Human Liver tissue lysate as well as PON1 recombinant protein.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Parkinson's disease
      • Other
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Hydrolysis
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Signal Transduction
      • Metabolism
      • Drug metabolism
      • Cell Biology
      • Other Antibodies
      • Oxidative Stress
      • Cardiovascular
      • Atherosclerosis
      • Diabetes associated
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Hydrolysis
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Drug metabolism
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Oxidative stress
      • Metabolism
      • Types of disease
      • Neurodegenerative disease
      • Metabolism
      • Types of disease
      • Diabetes
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human PON1 protein (ab53376)

    Applications

    Our Abpromise guarantee covers the use of ab99037 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

    Target

    • Function

      Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
    • Tissue specificity

      Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
    • Involvement in disease

      Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.
    • Sequence similarities

      Belongs to the paraoxonase family.
    • Post-translational
      modifications

      Glycosylated.
      The signal sequence is not cleaved.
      Present in two forms, form B contains a disulfide bond, form A does not.
    • Cellular localization

      Secreted > extracellular space.
    • Target information above from: UniProt accession P27169 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5444 Human
      • Entrez Gene: 18979 Mouse
      • Entrez Gene: 84024 Rat
      • Omim: 168820 Human
      • SwissProt: P27169 Human
      • SwissProt: P52430 Mouse
      • SwissProt: P55159 Rat
      • Unigene: 370995 Human
      • Unigene: 237657 Mouse
      • Unigene: 20732 Rat
      see all
    • Alternative names

      • A esterase 1 antibody
      • A-esterase 1 antibody
      • Aromatic esterase 1 antibody
      • Arylesterase 1 antibody
      • Arylesterase B type antibody
      • ESA antibody
      • Esterase A antibody
      • K 45 antibody
      • K-45 antibody
      • MVCD5 antibody
      • Paraoxonase 1 antibody
      • Paraoxonase antibody
      • Paraoxonase B type antibody
      • Paraoxonase, plasma antibody
      • Paraoxonase1 antibody
      • PON 1 antibody
      • PON antibody
      • PON1 antibody
      • PON1_HUMAN antibody
      • Serum aryldiakylphosphatase antibody
      • Serum aryldialkylphosphatase 1 antibody
      • Serum paraoxonase/arylesterase 1 antibody
      see all

    Images

    • Western blot - Anti-PON1 antibody (ab99037)
      Western blot - Anti-PON1 antibody (ab99037)
      All lanes : Anti-PON1 antibody (ab99037) at 1 µg/ml

      Lane 1 : Human liver tissue lysate - total protein (ab29889) at 10 µg
      Lane 2 : Recombinant Human PON1 protein (ab53376) at 0.1 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 39 kDa
      Observed band size: 39 kDa


      Exposure time: 3 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab99037? Please let us know so that we can cite the reference in this datasheet.

    ab99037 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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