Overview

  • Product name

    Anti-PON1 antibody [EPR2892]
    See all PON1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2892] to PON1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PON1 aa 50-150 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: plasma cells and ovary carcinoma lysate; IHC-P: liver tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab92466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
    IP: 1/10 - 1/100.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.

    Is unsuitable for ICC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
    • Tissue specificity

      Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
    • Involvement in disease

      Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.
    • Sequence similarities

      Belongs to the paraoxonase family.
    • Post-translational
      modifications

      Glycosylated.
      The signal sequence is not cleaved.
      Present in two forms, form B contains a disulfide bond, form A does not.
    • Cellular localization

      Secreted > extracellular space.
    • Information by UniProt
    • Database links

    • Alternative names

      • A esterase 1 antibody
      • A-esterase 1 antibody
      • Aromatic esterase 1 antibody
      • Arylesterase 1 antibody
      • Arylesterase B type antibody
      • ESA antibody
      • Esterase A antibody
      • K 45 antibody
      • K-45 antibody
      • MVCD5 antibody
      • Paraoxonase 1 antibody
      • Paraoxonase antibody
      • Paraoxonase B type antibody
      • Paraoxonase, plasma antibody
      • Paraoxonase1 antibody
      • PON 1 antibody
      • PON antibody
      • PON1 antibody
      • PON1_HUMAN antibody
      • Serum aryldiakylphosphatase antibody
      • Serum aryldialkylphosphatase 1 antibody
      • Serum paraoxonase/arylesterase 1 antibody
      see all

    Images

    • All lanes : Anti-PON1 antibody [EPR2892] (ab92466) at 1/1000 dilution

      Lane 1 : Human plasma cell lysate
      Lane 2 : ovarian cancer lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lane 1 : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
      Lane 2 : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 40 kDa

    • ab92466, at 1/100 dilution, staining PON1 in formalin-fixed, paraffin-embedded Human liver tissue.

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

       

    • ab92466 showing negative staining in Skeletal muscle tissue.

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

       

    • ab92466 showing negative staining in Normal colon tissue.

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

       

    • ab92466 showing negative staining in Normal breast tissue.

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • ab92466 showing negative staining in Normal brain tissue.

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

       

    References

    This product has been referenced in:

    • Yu Z  et al. Evaluation of the prognostic value of paraoxonase 1 in the recurrence and metastasis of hepatocellular carcinoma and establishment of a liver-specific predictive model of survival. J Transl Med 16:327 (2018). Read more (PubMed: 30477582) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for your patience. I have received some confirmation about some products and would like to update you on the information. ab85347: We have not tested this product for cross reactivity with PON2 and PON3. ab40775: We have not tested this product for cross reactivity with PON2 and PON3. ab53193: This antibody has been raised to a synthetic peptide corresponding to amino acids 298-309 of human PON1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence differs by one amino acid in mouse and two amino acids in rat. The peptide sequence differs by one amino acid in human PON2 and two amino acids in human PON3. Due to the homology between these two sequence, it is likely that some cross reactivity with PON2 and PON3 may occur. A 1 amino acid difference in the peptide would translate to 90% and 2 amino acid difference would be closer to 80% homology. Thus there is a less chance that PON3 would cross react. ab92466: We have not tested this product but there is 57.1% homology with PON2 (Q15165) and 50.0% identity with PON3. Therefore, we do not expect cross-reactivity with either one. I am still waiting for information on ab24261 and ab26930 and will be able to get back to you once I receive confirmation from the originator. Please bear in mind that all these products were not tested for cross reactivity with PON2 nor PON3. Therefore, we really do not know for sure. However, I can reassure you that all of our products are guaranteed to work in species and application as stated on the datasheet. Should you not get expected results, then please inform us within the guarantee period of 120 days (Abpromise). We will help troubleshoot the experiment and if the product is indeed faulty, we will be more than happy to offer a free replacement or refund. I hope our Abpromise guarantee will give you the assurance you need to use our products in your research. In the future, should you wish to contact us in Japanese, please feel free to do so as our system is able to accept both English and Japanese characters. We also have scientific support members in Japan who will be able to attend to your Japanese enquiries.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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