Recombinant
Recombinant Anti-PON1 antibody [EPR2893] (ab126597)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-PON1 antibody [EPR2893]
See all PON1 primary antibodies -
Description
Rabbit monoclonal [EPR2893] to PON1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PON1 aa 100-200. The exact sequence is proprietary.
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Positive control
- Human fetal kidney, ovary cancer, fetal liver and plasma lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2893 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for ICC or IHC-P.
Target
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Function
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. -
Tissue specificity
Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. -
Involvement in disease
Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. -
Sequence similarities
Belongs to the paraoxonase family. -
Post-translational
modificationsGlycosylated.
The signal sequence is not cleaved.
Present in two forms, form B contains a disulfide bond, form A does not. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 5444 Human
- Entrez Gene: 18979 Mouse
- Entrez Gene: 84024 Rat
- Omim: 168820 Human
- SwissProt: P27169 Human
- SwissProt: P52430 Mouse
- SwissProt: P55159 Rat
- Unigene: 370995 Human
see all -
Alternative names
- A esterase 1 antibody
- A-esterase 1 antibody
- Aromatic esterase 1 antibody
see all
Images
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![Western blot - Anti-PON1 antibody [EPR2893] (ab126597)](https://www.abcam.com/ps/products/126/ab126597/Images/ab126597-140553-anti-pon1-antibody-epr2893-western-blot.jpg)
Western blot - Anti-PON1 antibody [EPR2893] (ab126597)All lanes : Anti-PON1 antibody [EPR2893] (ab126597) at 1/10000 dilution
Lane 1 : Fetal kidney lysate
Lane 2 : Ovary cancer lysate
Lane 3 : Fetal liver lysate
Lane 4 : Human plasma lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Datasheets and documents
References
This product has been referenced in:
- Li Y et al. Hepatic Forkhead Box Protein A3 Regulates ApoA-I (Apolipoprotein A-I) Expression, Cholesterol Efflux, and Atherogenesis. Arterioscler Thromb Vasc Biol 39:1574-1587 (2019). Read more (PubMed: 31291759) »
- Huang Y et al. Myeloperoxidase, paraoxonase-1, and HDL form a functional ternary complex. J Clin Invest 123:3815-28 (2013). IP ; Human . Read more (PubMed: 23908111) »
