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    pon1-antibody-epr2893-ab126597.pdf

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RecombinantRabMAb

Recombinant Anti-PON1 antibody [EPR2893] (ab126597)

  • Datasheet
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Western blot - Anti-PON1 antibody [EPR2893] (ab126597)
  • Immunoprecipitation - Anti-PON1 antibody [EPR2893] (ab126597)
  • Anti-PON1 antibody [EPR2893] (ab126597)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2893] to PON1
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Primary
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Anti-ADAMTS7 antibody (ab203027)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human PON1 protein (ab53376)

View more associated products

Overview

  • Product name

    Anti-PON1 antibody [EPR2893]
    See all PON1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2893] to PON1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PON1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • IP: Human liver lysate. WB: Human mouse and rat plasma and liver lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2893
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Hydrolysis
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Hydrolysis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-PON1 antibody [EPR2893] - BSA and Azide free (ab248121)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human PON1 protein (ab53376)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/20.
WB
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Notes
IP
1/20.
WB
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

Target

  • Function

    Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
  • Tissue specificity

    Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
  • Involvement in disease

    Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.
  • Sequence similarities

    Belongs to the paraoxonase family.
  • Post-translational
    modifications

    Glycosylated.
    The signal sequence is not cleaved.
    Present in two forms, form B contains a disulfide bond, form A does not.
  • Cellular localization

    Secreted > extracellular space.
  • Target information above from: UniProt accession P27169 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5444 Human
    • Entrez Gene: 18979 Mouse
    • Entrez Gene: 84024 Rat
    • Omim: 168820 Human
    • SwissProt: P27169 Human
    • SwissProt: P52430 Mouse
    • SwissProt: P55159 Rat
    • Unigene: 370995 Human
    • Unigene: 237657 Mouse
    • Unigene: 20732 Rat
    see all
  • Alternative names

    • A esterase 1 antibody
    • A-esterase 1 antibody
    • Aromatic esterase 1 antibody
    • Arylesterase 1 antibody
    • Arylesterase B type antibody
    • ESA antibody
    • Esterase A antibody
    • K 45 antibody
    • K-45 antibody
    • MVCD5 antibody
    • Paraoxonase 1 antibody
    • Paraoxonase antibody
    • Paraoxonase B type antibody
    • Paraoxonase, plasma antibody
    • Paraoxonase1 antibody
    • PON 1 antibody
    • PON antibody
    • PON1 antibody
    • PON1_HUMAN antibody
    • Serum aryldiakylphosphatase antibody
    • Serum aryldialkylphosphatase 1 antibody
    • Serum paraoxonase/arylesterase 1 antibody
    see all

Images

  • Western blot - Anti-PON1 antibody [EPR2893] (ab126597)
    Western blot - Anti-PON1 antibody [EPR2893] (ab126597)
    All lanes : Anti-PON1 antibody [EPR2893] (ab126597) at 1/1000 dilution

    Lane 1 : Human plasma lysate
    Lane 2 : Human liver lysate
    Lane 3 : Mouse plasma lysate
    Lane 4 : Mouse liver lysate
    Lane 5 : Rat plasma lysate
    Lane 6 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40-45 kDa why is the actual band size different from the predicted?



    The expression profile and molecular weights observed are consistent with what have been described in the literatures (PMID: 17906223, 30262871).

  • Immunoprecipitation - Anti-PON1 antibody [EPR2893] (ab126597)
    Immunoprecipitation - Anti-PON1 antibody [EPR2893] (ab126597)

    Purified ab126597 at 1/20 dilution (0.5µg) immunoprecipitating PON1 in Human liver lysate.
    Lane 1 (input): Human liver lysate 10µg
    Lane 2 (+): ab126597 + Human liver lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126597 in Human liver lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 40-45 kDa

  • Anti-PON1 antibody [EPR2893] (ab126597)
    Anti-PON1 antibody [EPR2893] (ab126597)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab126597? Please let us know so that we can cite the reference in this datasheet.

ab126597 has been referenced in 2 publications.

  • Li Y  et al. Hepatic Forkhead Box Protein A3 Regulates ApoA-I (Apolipoprotein A-I) Expression, Cholesterol Efflux, and Atherogenesis. Arterioscler Thromb Vasc Biol 39:1574-1587 (2019). PubMed: 31291759
  • Huang Y  et al. Myeloperoxidase, paraoxonase-1, and HDL form a functional ternary complex. J Clin Invest 123:3815-28 (2013). IP ; Human . PubMed: 23908111

Customer reviews and Q&As

Show All Reviews Q&A
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Immunocytochemistry/ Immunofluorescence abreview for Anti-PON1 antibody [EPR2893]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Neuroblastoma cell line (N2A))
Permeabilization
Yes - 0,1% Triton X-100
Specification
Neuroblastoma cell line (N2A)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Mr. Łukasz Witucki

Verified customer

Submitted Mar 12 2021

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