Recombinant Anti-PON1 antibody [EPR2893] (ab126597)
![Western blot - Anti-PON1 antibody [EPR2893] (ab126597)](https://www.abcam.com/ps/products/126/ab126597/Images/ab126597-411492-anti-pon1-antibody-epr2893-westernblot-human-mouse-rat.jpg "Western blot - Anti-PON1 antibody [EPR2893] (ab126597)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2893] to PON1
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PON1 antibody [EPR2893]
See all PON1 primary antibodies -
Description
Rabbit monoclonal [EPR2893] to PON1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PON1 aa 100-200. The exact sequence is proprietary.
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Positive control
- IP: Human liver lysate. WB: Human mouse and rat plasma and liver lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2893 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/20.
|
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
|
| Notes |
|---|
|
IP
1/20. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
Target
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Function
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. -
Tissue specificity
Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. -
Involvement in disease
Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. -
Sequence similarities
Belongs to the paraoxonase family. -
Post-translational
modificationsGlycosylated.
The signal sequence is not cleaved.
Present in two forms, form B contains a disulfide bond, form A does not. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 5444 Human
- Entrez Gene: 18979 Mouse
- Entrez Gene: 84024 Rat
- Omim: 168820 Human
- SwissProt: P27169 Human
- SwissProt: P52430 Mouse
- SwissProt: P55159 Rat
- Unigene: 370995 Human
see all -
Alternative names
- A esterase 1 antibody
- A-esterase 1 antibody
- Aromatic esterase 1 antibody
see all
Images
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Western blot - Anti-PON1 antibody [EPR2893] (ab126597)All lanes : Anti-PON1 antibody [EPR2893] (ab126597) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human liver lysate
Lane 3 : Mouse plasma lysate
Lane 4 : Mouse liver lysate
Lane 5 : Rat plasma lysate
Lane 6 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?The expression profile and molecular weights observed are consistent with what have been described in the literatures (PMID: 17906223, 30262871).
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![Immunoprecipitation - Anti-PON1 antibody [EPR2893] (ab126597)](https://www.abcam.com/ps/products/126/ab126597/Images/ab126597-2-anti-pon1-antibody-epr2893-immunoprecipitation-liver-human.jpg)
Immunoprecipitation - Anti-PON1 antibody [EPR2893] (ab126597)Purified ab126597 at 1/20 dilution (0.5µg) immunoprecipitating PON1 in Human liver lysate.
Lane 1 (input): Human liver lysate 10µg
Lane 2 (+): ab126597 + Human liver lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126597 in Human liver lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 40-45 kDa -
![Anti-PON1 antibody [EPR2893] (ab126597)](https://www.abcam.com/ps/products/126/ab126597/Images/ab126597-4-benefits-of-recombinant-antibodies.png)
Anti-PON1 antibody [EPR2893] (ab126597)
Datasheets and documents
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Datasheet download
References (2)
ab126597 has been referenced in 2 publications.
- Li Y et al. Hepatic Forkhead Box Protein A3 Regulates ApoA-I (Apolipoprotein A-I) Expression, Cholesterol Efflux, and Atherogenesis. Arterioscler Thromb Vasc Biol 39:1574-1587 (2019). PubMed: 31291759
- Huang Y et al. Myeloperoxidase, paraoxonase-1, and HDL form a functional ternary complex. J Clin Invest 123:3815-28 (2013). IP ; Human . PubMed: 23908111
