Overview

  • Product name

  • Description

    Rabbit polyclonal to PON2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PON2 aa 79-107 (N terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    PDKPGGILMMDLKEEKPRARELRISRGFD


    Database link: Q15165

  • Positive control

    • IHC-P: Human small intestine and brain cortex tissues. WB: HepG2 whole cell lysate (ab7900). Flow cytometry: K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 39 kDa.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
  • Tissue specificity

    Widely expressed with highest expression in liver, lung, placenta, testis and heart.
  • Sequence similarities

    Belongs to the paraoxonase family.
  • Post-translational
    modifications

    The signal sequence is not cleaved.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A esterase 2 antibody
    • A-esterase 2 antibody
    • Aromatic esterase 2 antibody
    • Paraoxonase 2 antibody
    • Paraoxonase nirs antibody
    • PON 2 antibody
    • PON2 antibody
    • PON2_HUMAN antibody
    • Serum aryldialkylphosphatase 2 antibody
    • Serum paraoxonase/arylesterase 2 antibody
    see all

Images

  • Anti-PON2 antibody (ab230503) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg

    Predicted band size: 39 kDa

  • Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labelling PON2 using ab230503 at 1/10 dilution (green) compared to the negetive control (blue). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

  • Formalin-fixed, paraformaldehyde-embedded human brain cortex tissue stained for PON2 using ab230503 at 1/10 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraformaldehyde-embedded human small intestine tissue stained for PON2 using ab230503 at 1/10 dilution in immunohistochemical analysis.

References

ab230503 has not yet been referenced specifically in any publications.

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